Team:Edinburgh/Conclusions
From 2011.igem.org
(Difference between revisions)
Line 20: | Line 20: | ||
'''Does the BioSandwich DNA assembly method work properly?''' | '''Does the BioSandwich DNA assembly method work properly?''' | ||
+ | |||
+ | : Like other homology-based assembly methods, it produces [[Team:Edinburgh/Results#BioSandwich | both correct and incorrect assemblies]]. But we successfully used it to produce <partinfo>BBa_K523013</partinfo>. | ||
'''How would a biorefinery involving either system actually be constructed?''' | '''How would a biorefinery involving either system actually be constructed?''' | ||
+ | |||
+ | : Our [[Team:Edinburgh/Biorefinery | biorefinery]] page includes a process flow diagram that illustrates this. | ||
'''Would such a biorefinery be economically viable?''' | '''Would such a biorefinery be economically viable?''' | ||
+ | |||
+ | : Our [[Team:Edinburgh/Biorefinery | biorefinery]] page also attempts to answer this question. Such a biorefinery could indeed be economically viable. | ||
'''What are the social implications of creating such a biorefinery?''' | '''What are the social implications of creating such a biorefinery?''' |
Revision as of 10:47, 12 September 2011
In our abstract, we asked a number of questions that our feasibility study was intended to answer.
Would the efficiency of cellulases be increased by having different types close together?
- Very probably. Our C model and Kappa model showed that synergy makes sense in silico.
Could this be done by displaying them on a cell outer membrane?
- This seems a promising approach. <partinfo>BBa_K265008</partinfo> seems a promising carrier for placing enzymes at high copy number on the E. coli outer membrane.
Could it be done by displaying them on a phage?
- Further work is needed to answer this question. There are theoretical reasons to expect that this is a challenging problem.
Does the BioSandwich DNA assembly method work properly?
- Like other homology-based assembly methods, it produces both correct and incorrect assemblies. But we successfully used it to produce <partinfo>BBa_K523013</partinfo>.
How would a biorefinery involving either system actually be constructed?
- Our biorefinery page includes a process flow diagram that illustrates this.
Would such a biorefinery be economically viable?
- Our biorefinery page also attempts to answer this question. Such a biorefinery could indeed be economically viable.
What are the social implications of creating such a biorefinery?
What are people's thoughts and feelings regarding this project?
Should we (meaning society) actually build such a biorefinery?