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Team:Osaka/week7
From 2011.igem.org
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==September 11(Sun)== | ==September 11(Sun)== | ||
| + | # Gel extraction of digests | ||
| + | # Miniprep of yesterday's culture | ||
| + | # Restriction digests of minipreppped parts | ||
| + | # Gel electrophoresis of digests | ||
| + | # Ligation | ||
| + | #* 103(C):PprM+1-3A(vector) | ||
| + | #* 104(C):RecA+1-3A(vector) | ||
| + | #* 105(A):12(upstream)+101(downstream)+1-1G(vector) | ||
| + | #* 106(A):12(upstream)+102(downstream)+1-1G(vector) | ||
| + | # Transformation of ligation products | ||
Revision as of 09:29, 11 September 2011
Contents |
September 11(Sun)
- Gel extraction of digests
- Miniprep of yesterday's culture
- Restriction digests of minipreppped parts
- Gel electrophoresis of digests
- Ligation
- 103(C):PprM+1-3A(vector)
- 104(C):RecA+1-3A(vector)
- 105(A):12(upstream)+101(downstream)+1-1G(vector)
- 106(A):12(upstream)+102(downstream)+1-1G(vector)
- Transformation of ligation products
