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Team:Paris Bettencourt/T7 diffusion
From 2011.igem.org
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| + | <h1>T7 RNA polymerase</h1> | ||
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| + | <p>Bacteriophage T7 RNA polymerase is a DNA-dependent RNA polymerase from the T7 bacteriophage genome. The enzyme is composed of a single polypeptide chain of 880 amino acids. It catalyzes the processive polymerization of messenger RNA from nucleoside triphosphate precursors by using one strand of DNA as a template . This enzyme is known to have a stringent specificity for its promoter, that is orthogonal to the other promoterr of the cell.</p> | ||
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| + | <p>In our designs, we wanted a protein to pass through the tube and trigger a signal in the receiver cell. We see here that T7 RNA polymerase si a very good candidate for sugh system. That's why we used as <a href="https://2011.igem.org/Team:Paris_Bettencourt/Designs">one of our proof of principle designs.</a></p> | ||
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The diffusion of the T7 polymerase is the simplest design we are currently working on. | The diffusion of the T7 polymerase is the simplest design we are currently working on. | ||
Revision as of 13:29, 6 September 2011
T7 RNA polymerase
Bacteriophage T7 RNA polymerase is a DNA-dependent RNA polymerase from the T7 bacteriophage genome. The enzyme is composed of a single polypeptide chain of 880 amino acids. It catalyzes the processive polymerization of messenger RNA from nucleoside triphosphate precursors by using one strand of DNA as a template . This enzyme is known to have a stringent specificity for its promoter, that is orthogonal to the other promoterr of the cell.
In our designs, we wanted a protein to pass through the tube and trigger a signal in the receiver cell. We see here that T7 RNA polymerase si a very good candidate for sugh system. That's why we used as one of our proof of principle designs.
The diffusion of the T7 polymerase is the simplest design we are currently working on. The T7 polymerase, produced in the emitter cell, diffuses through the nanotubes and comes to activate the T7 amplifier in the receptor cell, triggering the signal response. This process is summed up in the draft below:
The cloning plan has been established and we are currently building this system
