Team:Minnesota/dingx055-08252011182540-165

__NOTOC__
{{Team:Minnesota/Top}}
<br>
==  Cloning #3 ==

'''Project:''' Surface Display

'''Date:''' Thu, 25 Aug 2011 18:25:40 GMT

'''Author:''' Viki Ding (dingx055)

'''Access:''' Public

'''Revision History:'''

:* Mon, 26 Sep 2011 18:30:49 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)<!-- Generated by Rtf2WikiMarkup - RTF to Wiki Markup Converter 1.0, Copyright © Phyrstorm Technologies, Inc. 2011 -->
<!-- Technology built on the Itenso Rtf Converter Framework -->
<nowiki>Ligation:</nowiki>



<nowiki>INP 50uL rxn</nowiki>

<nowiki>- vector (4.8 ug/mL): 20 uL</nowiki>

<nowiki>- INP (17.6 ug/mL): 22 uL</nowiki>

<nowiki>- ligase: 1 uL</nowiki>

<nowiki>- ligase buffer: 5 uL</nowiki>

<nowiki>- H2O: 2 uL</nowiki>



<nowiki>Negative control 30 uL rxn</nowiki>

<nowiki>- vector: 6 uL</nowiki>

<nowiki>- H2O: 20 uL</nowiki>

<nowiki>- ligase: 1 uL</nowiki>

<nowiki>- ligase buffer: 3 uL</nowiki>



<nowiki>Incubate at 16 degrees for 1 hr.</nowiki>



<nowiki>Transformation: see protocal 7/13/11</nowiki>



<nowiki>Single digestion of 8/24/11 clones</nowiki>

<nowiki>- 1 uL BamHI</nowiki>

<nowiki>- 3 buffer 4</nowiki>

<nowiki>- 26 uL cloned plasmid DNA</nowiki>



<nowiki>Incubate at 37 degrees overnight</nowiki>


{{Team:Minnesota/Bottom}}