Agenda: Establishment of the K346002 Hg Sensitive Promotor

===''' 05/24/11 Agenda: Establishment of the K346002 Hg Sensitive Promotor'''===

 (1)Electroporation of K346002 into Top 10 cells. 

 (2)40 microliters of Top 10 cells (JH 5-23-10)10E9 + 2 microliters K346002 DNA from Registry.

 (3)Parameters of electroporation: not recorded.

 (4)Added 1 microliter of SOB media (JH 2-11-11)immediately after electroporation.

 (5)Incubated tube at 37 degrees Celsius for 30 minutes.

 (6)Plated 200 microliters of transformation mixture on LB Agar AMP 5 micrograms/microliter 
   (4-21-10).Stored mixture at 4 degrees Celsius. 

  Expected: 10 to 50 colonies projected to grow.


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