Team:Amsterdam

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<h1>Welcome                            <g:plusone></g:plusone></h1>
Welcome to the <a href="https://2011.igem.org/Main_Page">iGEM</a> 2011 Amsterdam wiki!
Our <a href="https://2011.igem.org/Team:Amsterdam/Team/Students">team</a> consists of students from the <a href="http://www.english.uva.nl/start.cfm">University of Amsterdam</a> and the <a href="http://www.vu.nl/en/index.asp"> VU University Amsterdam</a>.
On this site you can find everything that has to do with our project: <a href="https://2011.igem.org/Team:Amsterdam/Project/Description"><b>icE. coli</b></a>. You will also find information on:<br><br>

<ul><li>Our <a href="https://2011.igem.org/Team:Amsterdam/Human_Outreach/Overview">human outreach</a>
<li>Our <a href="https://2011.igem.org/Team:Amsterdam/Sponsors/Companies">sponsors</a>
<li>The <a href="https://2011.igem.org/Team:Amsterdam/Notebook/Overview">day-to-day labwork</a><br>
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... and lots of other information relating to our project.<br><br>
<b>Want a quick no-nonsense tour of our project? Visit the <a href=https://2011.igem.org/Team:Amsterdam/Project/Data>data page</a>.</b>
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Please take a look around, and be sure to <A HREF="mailto:igemamsterdam@gmail.com">contact us</a> with any questions!
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<h1>Abstract</h1>
<i>Escherichia coli's</i> optimal growth temperature is 37°C. Growth rate decreases drastically at lower temperatures, and completely halts below 7°C. Our project aims to increase <i>E. coli’s</i> cold tolerance, by expressing several chaperone proteins normally found in psychrophillic bacteria. Chaperone proteins regulate structure of biological molecules, like proteins and RNA, in some cases safeguarding their function at low temperatures. By expressing psychrophillic chaperones, we aim to enhance <i>E. coli's</i> cold resistance in two ways. Growth rate is increased at temperatures below 37°C, possibly even enabling growth at 0°C, and <i>E. coli’s</i> ability to survive freezing environments is enhanced. Expression of psychrophile chaperones is achieved through BioBricks, combining promoters and ribosome binding sites characterised by previous iGEM teams with newly synthesized coding regions. Our team has successfully expressed two psychrophile chaperones in <i>E. coli</i>. We demonstrate CspC enhances the survival rate of frozen <i>E. coli</i>, and investigate putative novel functions of Cpn10. <br><br>
<h1>Medal requirements</h1>
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<td class="title">Bronze</td>
<td class="middletitle">Silver</td>
<td class="title">Gold</td>
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<td><a href="https://igem.org/Team.cgi?id=538"><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">Team registration</a></td>
<td class="middle"><a href="https://2011.igem.org/Team:Amsterdam/Labwork/Characterization"><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">Working BioBricks</a></td>
<td><a href="https://2011.igem.org/Team:Amsterdam/Human_Outreach/Collaboration"><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">Collaboration</a></td>
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<td><a href="https://igem.org/2011_Judging_Form?id=538"><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">Complete judging form</a></td>
<td class="middle"><a href="https://2011.igem.org/Team:Amsterdam/Labwork/Characterisation"><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">BioBrick characterization</a></td>
<td><a href="https://2011.igem.org/Team:Amsterdam/Human_Outreach/Overview"><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">Human outreach</a></td>
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<td><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">Team Wiki</td>
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<td><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">Present at iGEM Jamboree</td>
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<td><a href="https://2011.igem.org/Team:Amsterdam/Biobricks/Overview#Registry_Submissions"><img src="https://static.igem.org/mediawiki/2011/b/b1/Igemcheck.jpg">New BioBricks submitted</td>
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<h1>News</h1>
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Would you like to contact us? <A HREF="mailto:igemamsterdam@gmail.com">E-Mail</A>
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Would you like to help us?
<a href="http://www.indiegogo.com/iGEM-Amsterdam-icE-coli">Donate here!</a><br>
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