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Uppsala-Sweden/5 July 2011
From 2011.igem.org
Welcome to Uppsala-SwedeniEM '2011
Notebook
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2011-07-05
The day started of by running a PCR on the DNA template of the green light sensor which includes the two genes ccaR and ccaS as well as the promotor PcpcG2 (see protocol ccaR BioBrick, protocol ccaS BioBrick, protocol PcpcG2 BioBrick).
After lunch we did a plasmid preparation of the overnight cultures from 4/7 (purification protocol). At the end of the day we did a PCR to verify the red light sensor (cph8). We also did site specific mutagensis using PCR for the pigments amilGFP and amilCP in order to remove illegal EcoRI sites within the genes (protocol cph8, protocol amilGFP_EcoRI, protocol amilCP_EcoRI)
