Uppsala-Sweden/28 June 2011
From 2011.igem.org
Welcome to Uppsala-SwedeniEM '2011
Notebook
|
|
|
|
|
2011-06-28
Transformation of BioBrick plasmids (protocol for transforming TOP10 competent cells):
pSB1A3-J04450 (ampR backbone) pSB1C3-J04450 (CmR backbone) pSB1K3-J04450 (KanR backbone) pSB1AK3-B0014 (Double terminator) pSB1AK3-B1001 (synthetic terminator) pSB1A2-B0034 (Standard RBS) pSB2K3-I15008 (ho1, chromophore gene) pSB2K3-I15009 (pcyA, chomophore) pSB1A2-R0011 (PllacO, lacI repressable promotor) pSB2K3-I15010 (cph8 red sensor)