This page shows a list of all the parts that we have made or used in the project. Click on each link to see more details about that part on the Registry of Standard Biological Parts.

As our project focus on tor2 gene coming from yeast and contains common restriction sites like EcoRI, NotI and SpeI, the stantard Plasmid Backbone and BioBrick prefix/suffix is no longer appropriate. Furthermore, the size of 7800bp makes it pretty too expensive and inconvenient for chemical synthesis and codon optimization to avoid certain restriction sites. To match with the core gene and protein, a series of parts also give up standard prefix/suffix and backbones. We have used at least 3 kinds of plasmids, including blunt-end vectors to Yeast Surface Display Vector. However, based on the splendid standard plasmid backbone pSB1C3, which has a small size and simple MCS for easy-operation, we modified several vectors, which also render functionality and ease-of-use of promoter parts.

1. Inducible pCIT2 – Mut-TOR2 - Terminator, BBa_K563061: Promoter of CIT2 gene would be binding with transcription factor RTG1/3 at the presence of FAP. Then our reconstructed Mut-TOR2 would be expressed and forms in Mut-TORC1 which is not allergic to FAP. The accumulation of Mut-TORC1 will help the intracellular phosphorylation back to normal state.

2. pYE- Reporter, BBa_K563040 ~ BBa_K563046: We have successfully construct vectors, which coding bright fluorescent protein and Luciferase and beta-galactosidase in yeast cell. This series of BioBricks provide a convenient method for the measurement and detection of gene expression and terminate the unsuccessful expression of fluorescent protein in yeast cell.

3. Modified Plasmid Backbone – pSBY11, BBa_K563051: We reconstruct the Yeast Vector pYD through the combination with pSB1A11. According to specific primers, we amplify the yeast elements (CEN4/ARS6, TRP1 orf and terminator region) by PCR, and then insert the fragment into pSB1A11 by single restriction site PstI. In this way, a modified vector that combines pYD and pSB1A11 together(pSBY1) for large fragment transformation between yeast and E. coli is built.

	Name	Type	Description	Designer	Length
1	BBa_K563000	Regulatory	Promotor of HIS4	Boxuan Zeng	508
2	BBa_K563001	Regulatory	Promoter of DAL7	Boxuan Zeng	498
3	BBa_K563002	Regulatory	Promoter of ILV1	Boxuan Zeng	509
4	BBa_K563003	Regulatory	Promoter of CIT2	Boxuan Zeng	497
5	BBa_K563004	Regulatory	Promoter of TEF1	Boxuan Zeng	450
6	BBa_K563010	Coding	Original TOR	Boxuan Zeng	8118
7	BBa_K563011	Coding	TOR (LP Mutation)	Boxuan Zeng	8118
8	BBa_K563012	Coding	TOR (ITMutation)	Boxuan Zeng	8118
9	BBa_K563013	Coding	TOR (IT/EK Mutation)	Boxuan Zeng	8118
10	BBa_K563014	Coding	TOR (IT/VA Mutation)	Boxuan Zeng	8118
11	BBa_K563015	Coding	TOR (EK/VA Mutation)	Boxuan Zeng	8118
12	BBa_K563016	Coding	TOR (IT/VA/EK Mutation)	Boxuan Zeng	8118
13	BBa_K563030	Coding	pEASY-TOR01	Wenbin Kuang	3067
14	BBa_K563031	Coding	pEASY-TOR11	Wenbin Kuang	2521
15	BBa_K563032	Coding	pEASY-TOR22	Wenbin Kuang	2709
16	BBa_K563033	Coding	pEASY-TOR33	Wenbin Kuang	2499
17	BBa_K563034	Coding	pEASY-TOR22(LP Mutation)	Mingrui Zhao	2709
18	BBa_K563035	Coding	pEASY-TOR33(IT Mutation)	Mingrui Zhao	2499
19	BBa_K563036	Coding	pEASY-TOR33(VA Mutation)	Jing Guo	2499
20	BBa_K563037	Coding	pEASY-TOR33(EK Mutation)	Jing Guo	2499
21	BBa_K563040	Reporter	pYE-EGFP	Bin Jia	739
22	BBa_K563041	Reporter	pYE-ERFP	Bin Jia	744
23	BBa_K563043	Reporter	pYE-EYFP	Bin Jia	753
24	BBa_K563045	Reporter	pYE-OFL Orange Firefly Luciferase	Bin Jia	1656
25	BBa_K563046	Reporter	pYE-EPIC Firefly Luciferase	Bin Jia	1656
26	BBa_K563047	Reporter	pYE-EOFP	Bin Jia	744
27	BBa_K563048	Reporter	pYE-LacZ	Bin Jia	3000
28	BBa_K563051	Coding	TOR in pSBY1	Bin Jia	11914
29	BBa_K563052	Plasmid	pSBY11(pSB1A11-TRP1 CEN/ARS Yeast Shuttle Vector)	Bin Jia	3830
30	BBa_K563053	Plasmid Backbone	pYE	Bin Jia	5900
31	BBa_K563054	Plasmid Backbone	pYD	Bin Jia	5090
32	BBa_K563050	Plasmid Backbone	pSB1A11(Modified from pSB1A2)	Bin Jia	2090