Team:Peking R/Parts

From 2011.igem.org

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Parts of iGEM 2011 Peking_R

Favorite Parts

1.BBa_K598000

TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS+E0040+B0015

This part is TPP up-regulated hammerhead ribozyme, consisting of 131 nucleotides.

2.BBa_K598001

Theophylline Responsive Riboswitch 1G1 with Engineered RBS+GFP generator

This is a GFP generator regulated by theophylline responsive riboswitch. mRNA with a theophylline riboswitch in it responds to theophylline concerntration, producing different fluorescence strength of GFP. Regulated by different promoters, it would be used to demonstrate theophylline responsive curve, providing data for modeling.

3.BBa_K598002

Bistable Switch Mutant 68

This part is one of the mutation libraries of bistable switch modifying the ribosome binding site (RBS) of cI434 gene.

Parts Sandbox

1. BBa_K598003, BBa_K598004

TPP series ribocontroller

2. BBa_K598005, BBa_K598006

Theophylline series ribocontroller

3.BBa_K598007, BBa_K598008

Ribocontroller with GFP as reporter

4.BBa_K598009,BBa_K598010,BBa_K598011,BBa_K598012,BBa_K598013,BBa_K598014

Complete plasmid contained pBAD promoter, ribocontroller and GFP