"

From 2011.igem.org

Camille & Danyel

We first exrtacted from the overnight culture of S27 (cf Yesterday's page ) the PSB1C3 plasmid containing RFP.
We digested two of the miniprep with EcoR1 and Pst1 for 30mn in a waterbath at 37°C. We used Fastdigest enzymes.

We then run those two digestion product on a 1% gel and otained three bands : 1kb, 2kb and 3kb.
We extracted the 2kb band and purified it with a Qiagen kit.
In the same time we've run a gel with 1µL of the PCR product from last night PCR.

At this time, we were not very convinced of what we observed so to be sure to have something correct we launched again the same PCR as yesterday.

Discussion with the team the next morning show us that the results were ok.