HokkaidoU Japan

iGEM 2011 Team of Hokkaido University

Primers

Primer Name	Whole Sequence
Primer Name	F/R	Annealing Sequence	Tm	Adding Sequence

EX_F	gcagaattcgcggccgcttctagag
EX_F	Forward	Biobrick Prefix	74.5 C	None
PS_R	agcctgcagcggccgctactagta
PS_R	Reverse	Biobrick Suffix	74.6 C	None
suffix_F	tactagtagcggccgctgcaggct
suffix_F	Forward	Biobrick Suffix	74.6 C	None
prefix_R	ctctagaagcggccgcgaattctgc
prefix_R	Reverse	Biobrick Prefix	74.5 C	None
100up_EX_F	aacctataaaaataccgcatacac
100up_EX_F	Forward	100bp upstream from Biobrick prefix	62.7 C	None
200dn_PS_R	tcccctgattctgtggataaccgt
200dn_PS_R	Reverse	200bp downstream from Biobrick suffix	66.6 C	None

Xba_byebye_prefix_F	gcagaattcgcggccgcttcGagatg
Xba_byebye_prefix_F	Forward	Biobrick Prefix sequence	72.8 C	Single mutation T -> G in XbaI site.
EX_RBS_SlrP_F	GCAGAATTCGCGGCCGCTTCTAGAaaagaggagaaaatatgtttaatattactaatatacaatctacggc
EX_RBS_SlrP_F	Forward	RBS sequence, 5' terminal of SlrP coding sequence	69.7 C	Biobrick Prefix
PS_SlrP_R	AGCCTGCAGCGGCCGCTACTAGTggtaagtcctaatattttcagacgaag
PS_SlrP_R	Reverse	3'terminal of SlrP coding sequence	64.5 C	Biofusion Suffix
Bsa1_dt_F	GGCGACTAGAGAGACCccaggcatcaaataaaacgaaag
Bsa1_dt_F	Forward	5'terminal of double terminator sequence	63.6 C	BsaI recognition site and its cleavage site
Bsa1_SlrP_R	GGCCTGGCCTGAGACCCCggtaagtcctaatattttcagacga
Bsa1_SlrP_R	Reverse	3'terminal of SlrP coding Sequence	63.0 C	BsaI recognition site and its cleavage site
Bsa1_GSK_SlrP_R	GGCCTGGCCTGAGACCCCACTTTCAGCGAAACTTGTAGTGCGAGGGCGACCACTCATggtaagtcctaatattttcagacga
Bsa1_GSK_SlrP_R	Reverse	3'terminal of SlrP coding Sequence	63.6 C	GSK Tag, BsaI recognition site and its cleavage site