Materials and Equipment for Teachers considering.

From 2011.igem.org

Here is a list that helps you prepare for the iGEM competition. This list has the materials needed and the equipment that will be used in the iGEM competition. Some of the reagents are generic because the project will specify what type of reagent you will use. Also the list is divided by the type of organism you could use for the competition.

Yeast Materials: this list is a beginning point for the lab. There are protocols for Ecoli and Yeast on openwetware that are helpful. Growth media: YPD Selective media: SD with amino acids added Agar: YPD, Electrophoresis agar Restriction enzymes: Biobrick kit from New England Science will help Buffers: TAE/TBE/or LB Seed DNA Stains for gels Enzymes: DNA and RNA Polymerase (if needed), ligase, protease dNTP’s Chemicals: MgCl, Tris-HCl Equipment for the lab PCR, pressure cooker is being used as an autoclave, Micropipettes and tips, hemocytometer or spectrophotometer, Tubes, plates, Incubator, Shaking water bath or shaking incubator.

Ecoli Materials: this list is a beginning point for the lab. There are protocols for Ecoli and Yeast on openwetware that are helpful. Growth Media: T broth, M9, LB Selective Media: Antibiotics added to media Agar: LB agar, Electrophoresis agar Restriction enzymes: Biobrick kit from New England Science will help Buffers: TAE/TBE/or LB Stains for gels Enzymes: DNA and RNA Polymerase (if needed), ligase, protease dNTP’s Chemicals: MgCl, Tris-HCl

Equipment for the lab PCR, pressure cooker is being used as an autoclave, Micropipettes and tips, hemocytometer or spectrophotometer, Tubes, plates, Incubator, Shaking water bath or shaking incubator.