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From 2011.igem.org

Thursday

Lab work

• TLC on A1
• Membrane preparation on A2
• Run SDS-page on A2
• The bonds at the SDS-page from yesterday with A1 was unforunately not where the suppose to have been. Maybe there is a problem with the promoter we use...
• USER cloning with A2, B1, 2C9 and 1A2
• Transformation with orginal CYPs (before the mutations) to use for a control in next week

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