Thursday, July 21

From 2011.igem.org

(Difference between revisions)
 
(9 intermediate revisions not shown)
Line 1: Line 1:
-
8 mL of and 8 µL of  
+
==Tube Prep==
 +
Each tube contained 8 mL of LB broth and 8 µL of concentrated amphicillin 1000x.  In addition, each tube contained a single colony.  Each chosen colony was not near any other colonies to preserve diversity.
 +
These tubes were from the Promoter Plate
 +
*P1
 +
*P2
 +
*P3
 +
*P4
 +
These tubes were from the Terminator Plate
 +
*T1
 +
*T2
 +
*T3
 +
*T4
 +
These tubes were from the Ribosome Binding Site Plate
 +
*R1
 +
*R2
 +
*R3
 +
*R4
 +
===Incubation===
 +
The tubes were placed in the incubator at 4:21 PM at 31 Celsius and 135 RPM.  They should be removed in 24 hours, at 4:21 PM Friday.
 +
 +
 +
Dilution of 7-14-11 PCR products from lanes 1B,2B,4B, 5A, and 5B
 +
 +
:5.0  µl PCR reserved Product
 +
:12.5  µl 10x Buffer
 +
:107.5 µl Nuclease free water
 +
:'''125.0 µl Total'''
 +
--[[User:TravisCR|TravisCR]] 20:27, 21 July 2011 (CDT)
{{LabReportBmore}}
{{LabReportBmore}}

Latest revision as of 01:27, 22 July 2011

Tube Prep

Each tube contained 8 mL of LB broth and 8 µL of concentrated amphicillin 1000x. In addition, each tube contained a single colony. Each chosen colony was not near any other colonies to preserve diversity.


These tubes were from the Promoter Plate

  • P1
  • P2
  • P3
  • P4

These tubes were from the Terminator Plate

  • T1
  • T2
  • T3
  • T4

These tubes were from the Ribosome Binding Site Plate

  • R1
  • R2
  • R3
  • R4

Incubation

The tubes were placed in the incubator at 4:21 PM at 31 Celsius and 135 RPM. They should be removed in 24 hours, at 4:21 PM Friday.


Dilution of 7-14-11 PCR products from lanes 1B,2B,4B, 5A, and 5B

5.0 µl PCR reserved Product
12.5 µl 10x Buffer
107.5 µl Nuclease free water
125.0 µl Total

--TravisCR 20:27, 21 July 2011 (CDT) Team:Baltimore/Notebook

Hours