Team:ZJU-China/October.html

From 2011.igem.org

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</a> <a href="https://2011.igem.org/Team:ZJU-China/September.html">September<br />
</a> <a href="https://2011.igem.org/Team:ZJU-China/September.html">September<br />
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<a style="color:#0068b1" href="https://2011.igem.org/Team:ZJU-China/October.html">October</a></div>
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<a href="https://2011.igem.org/Team:ZJU-China/October.html">October</a></div>
<h4>Protocol</h4>
<h4>Protocol</h4>
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<td width="191">•Colony PCR</td>
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<td id="sheetleft">Oct.4th Tuesday</td>
<td id="sheetleft">Oct.4th Tuesday</td>
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<td >•Cut:RBS+20J<br/>•Cultivate:fdhf+2M-1,-2,-3,-4<br/>Vgb+2I-1,-222B+10I-1,-2<br/>•Ligate and purify:RBS+20J& 1C3 </td>
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<td >•Cut:RBS+20J<br/>•Ligate:2M+20J&10I to 1K3<br/>•Transform:2M+20J+10I,Vgb+1C3<br/>•Cultivate:22B+10I-1,-2</td>
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<td>•Miniprep and cut: fdhf+2M,Vgb+2I<br/>•Cut:fdhf+2M,20H</td>
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<td id="sheetleft">Oct.5th Wednesday</td>
<td id="sheetleft">Oct.5th Wednesday</td>
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<td >•Cultivate:Vgb+2I 1-6<br/>•Ligate:fdhf+20H& 1K3 </td>
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<td>•Transform:fdhf+20H<br/>•Cut:2M+20J</td>
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<td id="sheetleft">Oct.6th Thurday</td>
<td id="sheetleft">Oct.6th Thurday</td>
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<td>Finishing the wiki</td>
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<td >making poster</td>
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<td id="sheetleft">Oct.8th Saturday</td>
<td id="sheetleft">Oct.8th Saturday</td>
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<td >•Miniprep and test:<br/>Vgb,fdhf+20H+1K3 1-4</td>
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<td >•Colony PCR</td>
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<td id="sheetleft">Oct.9th Sunday</td>
<td id="sheetleft">Oct.9th Sunday</td>
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<td >•Digestion test and run the gel to test the results:<br/>20J+10I -1,-2,-4<br/>22B+10I -1,-2,-3,-4 Vgb+1C3<br/>Fdhf+2M+20H -1,-2,-3,-4 Vgb+2I -1,-2,-5</td>
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<td >•Colony PCR:fdhf+2M+20H 5-14<br/>•Cultivate:fdhf+2M+20H 5-14<br/>•Cut:fdhf+2M,20H</td>
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<td >•Ligate:Fdhf+2M&20H to 1C3<br/>•Cultivate:16L+Device C</td>
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<td>•Cut:Vgb+2I,22B+10I<br/>•Ligate:Vgb+2I&22B+10I to1C3</td>
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<td>•Cultivate:20H,22B+10I-1,20J+10I-1,-4 </td>
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<td>•Miniprep and cut:<br/>fdhf+2M+20H+1K3 5-14</td>
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<td id="sheetleft3">Oct.12th Wednesday</td>
<td id="sheetleft3">Oct.12th Wednesday</td>
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<td>•Miniprep: fdhf+20H-10,20H,22B+10I, 20J+10I-1,-4 fdhf+2I+20H,</td>
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                <td>•Cut and run the gel: Vgb+2I-5,<br/>22B+10I,20J+10I,1C3 backbone</td>
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<td id="sheetleft3">Oct.13th Thursday</td>
<td id="sheetleft3">Oct.13th Thursday</td>
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<td>•Cut and run the gel to test the ligate results</td>
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<td>•Regional Jamboree @ HKUST</td>
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<td>•Regional Jamboree @ HKUST</td>
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<td>•Regional Jamboree @ HKUST </td>
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<td>We are the Champion!!!</td>
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Revision as of 15:44, 26 October 2011

Notebook - September

Lab Notes

July August
 September
 October

Protocol

Protocol

Brainstorm

Brainstorm

Lab Notes - October

Biobrick Group

Week13

Day Note
Sept.26th Monday
•Miniprep: Rush-3,-4,-12
•Run the gel of digestion test results:
RFP+YFP+1K3-7,-16		 •PCR amplify: Rush-3,-4,-12
•Sample and test the intensity of fluorescence once or twice an hour during the growth of E.coli
Sept.27th Tuesday
•Ligate and transform:
RFP+YFP+CFP+1C3 RFP+YFP+1C3		 •PCR amplify:20H
•Cut: Rush Bone,22B
Sept.28th Wednesday
•Cultivate: DH5αwith Device CFP,RFP,YFP and without any device
•Ligate and purify:Rush+22B
•Cut and purify:
the product of the ligation		 •Run the gel: Digested Rush and 22B,Lig1
•Self-ligate:Digested Lig
•Colony PCR: RFP+YFP+CFP+1C3
•Cut and purify: RFP+YFP+1K3,12I
Sept.29th Thursday
•Ligate and transform: RFP+YFP+1K3&1C3,
RFP+YFP&1C3
•Colony PCR:RFP+YFP+CFP+1C3
•Preserve the colony on the plate
•Plate the ligation results: RFP+1C3,
RFP+YFP+CFP+1C3
Colony PCR:RFP+YFP+1C3
RFP+YFP+CFP+1C3
•Cultivate the above
Sept.30th Friday
•Colony PCR: Rush-Lig
Oct.1st Saturday
•Colony PCR: Failed
•MIniprep: 10I		 •Cut: 10I+22B,20J,1T3
•Ligate:10I+22B+1T3		 •Cultivate:2I,2M
Oct.2nd Sunday
•Transform the ligation results:
22B+10I(1T3),20J+10I(1T3)
•Miniprep and Preserve:2M,2I		 •Cut:fdhf,Vgb,2M,2I
•Purify:RFP+YFP+CFP+1C3		 •Ligate and transform:
RFP+YFP+CFP&1A3k
fdhf+2M&1K3,Vgb+2I&1K3

Week14

Day Note
Oct.3rd Monday
•Colony PCR
Oct.4th Tuesday
•Cut:RBS+20J
•Cultivate:fdhf+2M-1,-2,-3,-4
Vgb+2I-1,-222B+10I-1,-2
•Ligate and purify:RBS+20J& 1C3 		•Cut:RBS+20J
•Ligate:2M+20J&10I to 1K3
•Transform:2M+20J+10I,Vgb+1C3
•Cultivate:22B+10I-1,-2		 •Miniprep and cut: fdhf+2M,Vgb+2I
•Cut:fdhf+2M,20H
Oct.5th Wednesday
•Cultivate:Vgb+2I 1-6
•Ligate:fdhf+20H& 1K3 		•Transform:fdhf+20H
•Cut:2M+20J
Oct.6th Thurday
Finishing the wiki
Oct.7th Friday
making poster
Oct.8th Saturday
•Miniprep and test:
Vgb,fdhf+20H+1K3 1-4		 •Colony PCR
Oct.9th Sunday
•Digestion test and run the gel to test the results:
20J+10I -1,-2,-4
22B+10I -1,-2,-3,-4 Vgb+1C3
Fdhf+2M+20H -1,-2,-3,-4 Vgb+2I -1,-2,-5		 •Colony PCR:fdhf+2M+20H 5-14
•Cultivate:fdhf+2M+20H 5-14
•Cut:fdhf+2M,20H

 

Week15

Day Note
Oct.10th Monday
•Ligate:Fdhf+2M&20H to 1C3
•Cultivate:16L+Device C		 •Cut:Vgb+2I,22B+10I
•Ligate:Vgb+2I&22B+10I to1C3
Oct.11th Tuesday
•Cultivate:20H,22B+10I-1,20J+10I-1,-4 •Miniprep and cut:
fdhf+2M+20H+1K3 5-14
Oct.12th Wednesday
•Miniprep: fdhf+20H-10,20H,22B+10I, 20J+10I-1,-4 fdhf+2I+20H, •Cut and run the gel: Vgb+2I-5,
22B+10I,20J+10I,1C3 backbone
Oct.13th Thursday
•Cut and run the gel to test the ligate results
Oct.14th Friday
•Regional Jamboree @ HKUST
Oct.15th Saturday
•Regional Jamboree @ HKUST
Oct.16th Sunday
•Regional Jamboree @ HKUST We are the Champion!!!

 

Week16

Day Note
Oct.17th Monday
Oct.18th Tuesday
Oct.19th Wednesday
Oct.20th Thursday
Oct.21st Friday
Oct. 22nd Saturday
Oct.23rd Sunday

Week17

Day Note
Oct.24th Monday
Oct.25th Tuesday
Oct.26th Wednesday
Oct.27th Thursday
Oct.28th Friday
Oct. 29th Saturday
Oct.30th Sunday


Biofilm Group

3rd Oct

4th Oct

6th Oct

12th Oct

15th Oct

16th Oct

17th Oct

20th Oct

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