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Team:Yale/Notebook/Week6
From 2011.igem.org
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| - | Week 6 | + | <h1>Week 6: June 19-June 26</h1> |
| - | ---- | + | <ul> |
| - | + | <li>Cloning struggles: | |
| - | + | <ul> | |
| + | <li>Multiple attempts to PCR amplify ZeAFP with Taq and Phusion polymerase failed</li> | ||
| + | <li>Eventually succeed with Taq, digest with XbaI and SpeI</li> | ||
| + | <li>Ligate with T4 DNA ligase</li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li>Survival assays: | ||
| + | <ul><li>Completed survival assays on induced and uninduced MBP-TmAFP, eGFP-TmAFP, eGFP-TEV-TmAFP samples to determine greatest freeze-thaw viability (see figure:)</li> | ||
| + | <li>insert figure</li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | </ul> | ||
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Revision as of 23:30, 27 September 2011
