Team:Yale/Notebook/Week6

From 2011.igem.org

iGEM Yale

Week 6: June 19-June 26

  • Cloning struggles:
    • Multiple attempts to PCR amplify ZeAFP with Taq and Phusion polymerase failed
    • Eventually succeed with Taq, digest with XbaI and SpeI
    • Ligate with T4 DNA ligase
  • Survival assays:
    • Completed survival assays on induced and uninduced MBP-TmAFP, eGFP-TmAFP, eGFP-TEV-TmAFP samples to determine greatest freeze-thaw viability (see assays page)
    • More detailed descriptions in physical lab notebook