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Team:Yale/Notebook/Week6
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| - | <li><a | + | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week1">Week 1</a></li> |
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week2">Week 2</a></li> | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week2">Week 2</a></li> | ||
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week3">Week 3</a></li> | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week3">Week 3</a></li> | ||
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week4">Week 4</a></li> | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week4">Week 4</a></li> | ||
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week5">Week 5</a></li> | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week5">Week 5</a></li> | ||
| - | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week6">Week 6</a></li> | + | <li><a id="page" href="https://2011.igem.org/Team:Yale/Notebook/Week6">Week 6</a></li> |
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week7">Week 7</a></li> | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week7">Week 7</a></li> | ||
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week8">Week 8</a></li> | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week8">Week 8</a></li> | ||
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week9">Week 9</a></li> | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week9">Week 9</a></li> | ||
<li><a href="https://2011.igem.org/Team:Yale/Notebook/Week10">Week 10</a></li> | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week10">Week 10</a></li> | ||
| + | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week11">Week 11</a></li> | ||
| + | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week12">Week 12</a></li> | ||
| + | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week13">Week 13</a></li> | ||
| + | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week14">Week 14</a></li> | ||
| + | <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week15">Week 15</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
<div id="entry"> | <div id="entry"> | ||
| - | Week 6 | + | <h1>Week 6: June 19-June 26</h1> |
| - | ---- | + | <ul> |
| - | + | <li>Cloning struggles: | |
| - | + | <ul> | |
| + | <li>Multiple attempts to PCR amplify ZeAFP with Taq and Phusion polymerase failed</li> | ||
| + | <li>Eventually succeed with Taq, digest with XbaI and SpeI</li> | ||
| + | <li>Ligate with T4 DNA ligase</li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | <li>Survival assays: | ||
| + | <ul><li>Completed survival assays on induced and uninduced MBP-TmAFP, eGFP-TmAFP, eGFP-TEV-TmAFP samples to determine greatest freeze-thaw viability (see assays page)</li> | ||
| + | <li>More detailed descriptions in physical lab notebook</li> | ||
| + | </ul> | ||
| + | </li> | ||
| + | </ul> | ||
</div> | </div> | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
</div> | </div> | ||
Latest revision as of 01:35, 29 September 2011
