Team:Washington/Protocols/Gib Rxn
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Revision as of 03:59, 14 September 2011
Gibson Cloning/Assembly
- Add 15uL of Gibson MasterMix to each tube.
- Add ~20-30 ng of purified Backbone DNA to the reaction tube.
- Add ~20-30 ng of purified Insert DNA to the reaction tube.
- Fill the remaining tubes with distilled H20 to ensure a final volume of 20 uL
- After making sure all tubes are appropriately labeled, incubate all samples for ~1 hour @ 50oC.