From 2011.igem.org

Revision as of 03:27, 23 September 2011

An Overview of the 2011 UW iGEM Teams Summer Projects

Make It: Diesel Production Insert brief explanation of figure above here

Break It: Gluten Destruction Insert brief explanation of figure above here

The GibsonBricks ToolKit We completed two iGEM toolkits. In the first we created five Gibson Cloning versions of traditional biobrick vectors, and in the second we cloned the genes essential for magnetosome formation and transformed two into E. coli

Data Summary

Data for Favorite New Parts

Fill Me In.. here is an example:

1. Main Page - BAR responsive promoter, BBa_X0X0X: promoter can be induced to express a marker gene (GFP) when exposed to skunk smell (Butanethiol, C₄H₉SH), but not when exposed to pleasant scents
2. Main Page - Insulated Vector, BBa_XXX00: un-induced leaky expression is blocked by transcriptional terminators
3. Main Page - BAR Bad Odor Receptor, BBa_000XX: yellow fluorescent protein-tagged BAR (BBa_X00XX) shows that BAR is produced in E. coli

Data for Existing Parts

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1. Experience - Wintergreen odor enzyme generator, BBa_J45119 (MIT, iGEM 2006): 98 out of 100 volunteer subjects standing up to 5 feet away from the bacterial cultures could distinguish wintergreen-producing bacteria from negative controls.
2. Experience - RBS, BBa_J61110 (Arkin Lab, 2007): Of the 5 RBS Parts we tested, this RBS works best for expressing yellow fluorescent protein-tagged BAR

Improved Parts

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1. Main Page - Air Freshilizor, BBa_XXXXX: Our mathematical model predicts that the threshold of activation is 10 parts per billion, the concentration of Butanethiol that humans can typically smell

All Submitted Parts

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