Team:Washington/Magnetosomes/Parts

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iGEM Toolkits: Parts Submitted


iGem Toolkits: submitted a total twenty-two parts to the registry:

  • 10 magnetosome gene-groups,
  • 2 mam gene-sfGFP fusions,
  • 3 parts of the essential magnetosome gene- assembly, and
  • 5 pGA vectors.

Magnetosome Toolkit Favorite Parts

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590015 BBa_K590015 sfGFP_mamK_pGA1C3]

This part consists of mamK gene from Magnetospirillum magneticum strain AMB-1, sfGFP in the backbone of pGA1C3. mamK was previously reported for its importance proper magnetosome chain organization in magnetic bacteria; it is bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain.

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K590016 BBa_K590016 sfGFP_mamI_pGA1C3]

This part consists of mamI gene from Magnetospirillum magneticum strain AMB-1, sfGFP in the backbone of pGA1C3. MamI is a membrane-localized protein that localizes magnetic vesicles to the surface of cells, thus forming characteristic magnetosome chains.

The Gibson Assembly Toolkit

Gene AMB Number Cluster Membership Member of 28 genes list? (specific*/related**) Function Summary (Vesicle chain formation, and/or biomineralization) Gene Function
mamH amb0961 mamAB Related
mamI amb0962 mamAB Specific Vesicle, (Chain Formation?) >berkeley 2010: Loss causes no membrane formation, is localized onto chains
mamE amb0963 mamAB; mam Islet Related >Membrane-bound serine protease required for magnetite formation; might control the localization of other magnetosome proteins
mamJ amb0964 mamAB; mam Islet Specific Chain Formation >Proper magnetosome chain organization/assembly
mamK amb0965 mamAB; mam Islet Related Chain Formation >required for proper magnetosome chain organization; *bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain, shown to localize the mamI
mamL amb0966 mamAB; mam Islet Specific Vesicle, biomineralization >berkely 2010: Crucial to mangneosome membrane creation, shown to be spread across the cell membrane and sometimes forms lines
mamM amb0967 mamAB Related >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome
mamN amb0968 mamAB Related >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome
mamO amb0969 mamAB Related >biomineralization, involved in iron transport, magnetite nucleation, or establishement of the proper chemical enviornment for magnetite synthesis in the magnetosome
mamP amb0970 mamAB Related Biomineralization >berkeley 2010: loss causes weak magnetic response, with large but fewer crystals
mamA amb0971 mamAB Related >Required for magnetosome activation; activation of vessicles
mamQ amb0972 mamAB; mam Islet Related >ORF; formation/maintenance of magnetosome membranes
mamR amb0973 mamAB Specific Chain formation, Biomineralization >ORF; plays a role in controlling both particle number and size of magnetite cyrstals
mamB amb0974 mamAB Related Vesicle, Biomineralization >indirect role in magnetosome membrane invagination and biomineralization; magnetosome compartment formation
mamS amb0975 mamAB Specific
mamT amb0976 mamAB Specific Biomineralization >magnetite crystal growth; participates in different steps during magnetite synthesis
mamU amb0977 mamAB Related
mamV amb0978 mamAB N/A