Team:Washington/Magnetosomes/Background

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iGEM Toolkits

iGEM teams have started to investigate the possibility to make multiple-gene insert for their project as to bring the cloning project from single gene level to multiple gene level. However, current Biobrick Standard vectors are not designed for multiple-insert cloning. Thus, UW IGEM team decided to research on methods to improve the cloning efficiency and two "toolkits" were brought to the Register as a result.


Gibson Assembly Toolkit

As a continuation of 2010 UW IGEM project, this year we created and submitted several plasmid backbones that are Gibson cloning method friendly aka pGA vectors. It is called the Gibson Assembly Toolkit


Igem2011 GibsonToolkit.png

What's in the Gibson Assembly Toolkit?

  • Five plasmid backbones
  • pGA1A3, pGA1C3, pGA3K3, pGA4A5, pGA4C5









Magnetosome Toolkit

We are ambitious about making large construct that over several thousands base pairs. Therefore, utilizing the pGA vectors and Gibson cloning methods, the Magnetosome Toolkit was developed with the goal to build magnetic E.Coli, a characteristic that was not expressed in E.coli before.


Igem2011 MagnetToolkit.png

What’s in the Magnetosome Toolkit?

  • A set of 18 genes for the biominieralization of magnetic crystals
  • Our favorite genes in pGA vectors
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