Team:Washington/Magnetosomes/Background

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Revision as of 17:56, 22 September 2011 by Saushun (Talk | contribs)


iGEM Toolkits

iGEM teams have started to investigate the possibility to make multiple-gene insert for their project as to bring the cloning project from single gene level to multiple gene level. However, current Biobrick Standard vectors are not designed for multiple-insert cloning. Thus, UW IGEM team decided to research on methods to improve the cloning efficiency and two "toolkits" were brought to the Register as a result.

(icon of a "toolkit" )

Gibson Vector Toolkit

As a continuation of 2010 UW IGEM project, we created and submitted several plasmid backbones that are Gibson Cloning Friendly a.k.a pGA vectors.

They are pGA1A3, pGA1C3, pGA3K3, pGA4A5, pGA4C5

Magnetosome Toolkit

Igem2011 MagnetToolkit.png] ...What’s in the Magnetosome Toolkit… -A set of 18 genes for the biominieralization of magnetic crystals -Our favorite genes in pGA vectors