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Team:Washington/Magnetosomes/Background
From 2011.igem.org
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== iGEM Toolkits == | == iGEM Toolkits == | ||
| - | iGEM teams have started to investigate the possibility to make multiple-gene insert for their project as to bring the cloning project from single gene level to multiple gene level. However, current Biobrick Standard vectors are not designed for multiple-insert cloning. Thus, UW IGEM team decided to research on methods to improve the | + | iGEM teams have started to investigate the possibility to make multiple-gene insert for their project as to bring the cloning project from single gene level to multiple gene level. However, current Biobrick Standard vectors are not designed for multiple-insert cloning. Thus, UW IGEM team decided to research on methods to improve the cloning efficiency and two "toolkits" were brought to the Register as a result. |
(icon of a "toolkit" ) | (icon of a "toolkit" ) | ||
== Gibson Vector Toolkit == | == Gibson Vector Toolkit == | ||
| - | As a continuation of | + | As a continuation of [https://2010.igem.org/Team:Washington/Tools_Used/Next-Gen_Cloning 2010 UW IGEM project], we created and submitted several plasmid backbones that are Gibson Cloning Friendly a.k.a pGA vectors. |
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| + | They are pGA1A3, pGA1C3, pGA3K3, pGA4A5, pGA4C5 | ||
== Magnetosome Toolkit == | == Magnetosome Toolkit == | ||
| - | .. | + | [[File:Igem2011 MagnetToolkit.png|200px]]] |
| + | ...What’s in the Magnetosome Toolkit… | ||
| + | -A set of 18 genes for the biominieralization of magnetic crystals | ||
| + | -Our favorite genes in pGA vectors | ||
Revision as of 17:56, 22 September 2011
iGEM Toolkits
iGEM teams have started to investigate the possibility to make multiple-gene insert for their project as to bring the cloning project from single gene level to multiple gene level. However, current Biobrick Standard vectors are not designed for multiple-insert cloning. Thus, UW IGEM team decided to research on methods to improve the cloning efficiency and two "toolkits" were brought to the Register as a result.
(icon of a "toolkit" )
Gibson Vector Toolkit
As a continuation of 2010 UW IGEM project, we created and submitted several plasmid backbones that are Gibson Cloning Friendly a.k.a pGA vectors.
They are pGA1A3, pGA1C3, pGA3K3, pGA4A5, pGA4C5
Magnetosome Toolkit
]
...What’s in the Magnetosome Toolkit…
-A set of 18 genes for the biominieralization of magnetic crystals
-Our favorite genes in pGA vectors
