"
Team:Washington/Magnetosomes
From 2011.igem.org
| Line 4: | Line 4: | ||
<h1> | <h1> | ||
| + | Magnetosomes and Gibson Cloning | ||
| + | </h1> | ||
| + | |||
| + | <h2> | ||
Project goal: | Project goal: | ||
| - | </ | + | </h2> |
| - | <p | + | |
| + | <p> | ||
The goal of this project was to use gibson cloning to transfer the genes involved in magnetosome synthesis, into E.Coli. | The goal of this project was to use gibson cloning to transfer the genes involved in magnetosome synthesis, into E.Coli. | ||
</p> | </p> | ||
| - | < | + | |
| + | <h2> | ||
What are magnetosomes? <br /> | What are magnetosomes? <br /> | ||
| - | </ | + | </h2> |
| + | |||
<p> | <p> | ||
Magnetosomes are small invaginations of the bacterial cell membrane that contain magnetite nanoparticles. These particles range in size between 20 and several hundred nanometers and are aligned in one or several chains along the long axis of the bacteria. <br /> | Magnetosomes are small invaginations of the bacterial cell membrane that contain magnetite nanoparticles. These particles range in size between 20 and several hundred nanometers and are aligned in one or several chains along the long axis of the bacteria. <br /> | ||
| Line 17: | Line 24: | ||
These particles act together to form a magnetic dipole across the bacteria, allowing it to perceive the earths magnetic field. In the northern hemisphere, magnetotactic bacteria (bacteria with magnetosomes) swim north along the earths magnetic field lines in search of a micro-environement with a specific oxygen content. It is believed that the magnetosomes help bacteria turn the search for this perfect oxygen level from a three dimensional one (in all directions) to a one dimensional one along a single path. <br /> | These particles act together to form a magnetic dipole across the bacteria, allowing it to perceive the earths magnetic field. In the northern hemisphere, magnetotactic bacteria (bacteria with magnetosomes) swim north along the earths magnetic field lines in search of a micro-environement with a specific oxygen content. It is believed that the magnetosomes help bacteria turn the search for this perfect oxygen level from a three dimensional one (in all directions) to a one dimensional one along a single path. <br /> | ||
<br /> | <br /> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
</p> | </p> | ||
Revision as of 20:49, 5 September 2011
Magnetosomes and Gibson Cloning
Project goal:
The goal of this project was to use gibson cloning to transfer the genes involved in magnetosome synthesis, into E.Coli.
What are magnetosomes?
Magnetosomes are small invaginations of the bacterial cell membrane that contain magnetite nanoparticles. These particles range in size between 20 and several hundred nanometers and are aligned in one or several chains along the long axis of the bacteria.
These particles act together to form a magnetic dipole across the bacteria, allowing it to perceive the earths magnetic field. In the northern hemisphere, magnetotactic bacteria (bacteria with magnetosomes) swim north along the earths magnetic field lines in search of a micro-environement with a specific oxygen content. It is believed that the magnetosomes help bacteria turn the search for this perfect oxygen level from a three dimensional one (in all directions) to a one dimensional one along a single path.
