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Team:Washington/Alkanes/Future/Vector
From 2011.igem.org
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=Growth Optimization= | =Growth Optimization= | ||
| - | Using our initial, non-optimized growth conditions, we were able to obtain alkane/ene yields of approximately 2 mg/L. | + | Using our initial, non-optimized growth conditions, we were able to obtain alkane/ene yields of approximately 2 mg/L. In order to make analysis easier, and to make it easier to determine the effects of the addition of additional modules, we wanted to increase yield. Our efforts focused on how by varying system conditions, we could increase yield. |
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==Effect of Trace metals on yield== | ==Effect of Trace metals on yield== | ||
Revision as of 05:15, 17 September 2011
Growth Optimization
Using our initial, non-optimized growth conditions, we were able to obtain alkane/ene yields of approximately 2 mg/L. In order to make analysis easier, and to make it easier to determine the effects of the addition of additional modules, we wanted to increase yield. Our efforts focused on how by varying system conditions, we could increase yield.
Effect of Trace metals on yield
We had suspected that the trace metals in the original media may be inhibiting alkane production. We analyzed alkane production of cells transformed with the basic Petrobrick in medias of varying trace metal concentration.
Based upon this test, we determined that trace metals have a negative effect on alkane yield, and that all future tests would be conducted in an M9 media without additional trace metals.
Use of Different straings
We had suspected that different strains of E. coli would produce varying amounts of alkane. Initial experiments were done in MG1655, and we decided to test XL-1 blue( a commercial supercopentent variant of DH5a) for the ability to produce alkane.
XL-1 blue was able to produce more alkane than MG1655. In addition, XL-1 blue did not produce detectable levels of C16 alcohol. This makes C17 alkene analysis easier, and would mean a purer alkane product.
Covered vs Open
Aerobic vs Microaerobic
1C3 vs 3D4R
TB vs M9
and more...
