From 2011.igem.org

Localization

To improve efficiency of catalysis from acyl-ACPs through a two-step pathway to alkane production, we chose to consider optimization by protein co-localization. By decreasing the distance between Acyl-ACP Reductase (AAR) and Aldehyde Decarbonylase (ADC) proteins, we expect to improve alkane production concentrations.

Direct Fusion

Our first approach was direct fusion of two proteins using a glycine-serine linker. We chose to construct both configurations of AAR to ADC linkage to test the effects of fusion by N and C terminus regions on enzyme performance, resulting in both an AAR to ADC and an ADC to AAR fusion.

Zinc Finger Fusion