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Team:Washington/Alkanes/Future/Localization
From 2011.igem.org
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To improve efficiency of catalysis from acyl-ACPs through a two-step pathway to alkane production, we chose to consider optimization by protein co-localization. By decreasing the distance between Acyl-ACP Reductase (AAR) and Aldehyde Decarbonylase (ADC) proteins, we expect to improve alkane production. | To improve efficiency of catalysis from acyl-ACPs through a two-step pathway to alkane production, we chose to consider optimization by protein co-localization. By decreasing the distance between Acyl-ACP Reductase (AAR) and Aldehyde Decarbonylase (ADC) proteins, we expect to improve alkane production. | ||
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==Direct Fusion== | ==Direct Fusion== | ||
Our first approach was direct fusion of two proteins using a glycine-serine linker. We chose to construct both configurations of AAR to ADC linkage to test the effects of fusion by N and C terminus regions on enzyme performance, resulting in both an AAR to ADC and an ADC to AAR fusion. | Our first approach was direct fusion of two proteins using a glycine-serine linker. We chose to construct both configurations of AAR to ADC linkage to test the effects of fusion by N and C terminus regions on enzyme performance, resulting in both an AAR to ADC and an ADC to AAR fusion. | ||
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==Zinc Finger Fusion== | ==Zinc Finger Fusion== | ||
Revision as of 18:35, 12 September 2011
Localization
To improve efficiency of catalysis from acyl-ACPs through a two-step pathway to alkane production, we chose to consider optimization by protein co-localization. By decreasing the distance between Acyl-ACP Reductase (AAR) and Aldehyde Decarbonylase (ADC) proteins, we expect to improve alkane production.
Direct Fusion
Our first approach was direct fusion of two proteins using a glycine-serine linker. We chose to construct both configurations of AAR to ADC linkage to test the effects of fusion by N and C terminus regions on enzyme performance, resulting in both an AAR to ADC and an ADC to AAR fusion.
