Team:Washington/Alkanes/Future

From 2011.igem.org

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::Our current in vivo system only efficiently makes C15 alkanes. To be efficient enough for factory production, there are two broad goals to be done: 1. increase production efficiency 2. Diversity the range of alkanes for the system. 3. Increase scale of system for industrial processes. We have already begun efforts to expand the efficiency and scope of alkane production.
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We have begun efforts to expand the scope and effciency of alkane production. More information can be found in the following pages:
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[https://2011.igem.org/Team:Washington/Alkanes/Future/DecarbDesign Decarbonylase Redesign]  
[https://2011.igem.org/Team:Washington/Alkanes/Future/DecarbDesign Decarbonylase Redesign]  
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:<nowiki>One way to diversify the kind of alkanes produced is to alter the substrate specificity of the proteins involved. We decided to alter the aldehyde decarbonylase to produce shorter-chain fatty aldehydes.</nowiki>
[https://2011.igem.org/Team:Washington/Alkanes/Future/LuxCDE Alternate Aldehyde Production]
[https://2011.igem.org/Team:Washington/Alkanes/Future/LuxCDE Alternate Aldehyde Production]
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:<nowiki>Another way to diversify our system is to use alternative proteins. Our current system uses acyl-ACP reductase, and we've identified an hypothetical alternative system that produces aldehydes: LuxCDE, made from parts from the 2010
[https://2011.igem.org/Team:Washington/Alkanes/Future/FabH2 Branched Alkanes Production]
[https://2011.igem.org/Team:Washington/Alkanes/Future/FabH2 Branched Alkanes Production]

Revision as of 03:30, 16 September 2011


Our current in vivo system only efficiently makes C15 alkanes. To be efficient enough for factory production, there are two broad goals to be done: 1. increase production efficiency 2. Diversity the range of alkanes for the system. 3. Increase scale of system for industrial processes. We have already begun efforts to expand the efficiency and scope of alkane production.

Decarbonylase Redesign

One way to diversify the kind of alkanes produced is to alter the substrate specificity of the proteins involved. We decided to alter the aldehyde decarbonylase to produce shorter-chain fatty aldehydes.

Alternate Aldehyde Production

Another way to diversify our system is to use alternative proteins. Our current system uses acyl-ACP reductase, and we've identified an hypothetical alternative system that produces aldehydes: LuxCDE, made from parts from the 2010 [https://2011.igem.org/Team:Washington/Alkanes/Future/FabH2 Branched Alkanes Production] [https://2011.igem.org/Team:Washington/Alkanes/Future/Vector System Optimization] [https://2011.igem.org/Team:Washington/Alkanes/Future/Localization Enzyme Localization] [https://2011.igem.org/Team:Washington/Alkanes/Future/Yeast Alternative Chasis] [[Image:UW_2011_Alkane_FutureDirections_A.png|900px||center]] INSERT IN THESE PAGES... LOOK AT OTHER PAGES FOR EXAMPLES OF FORMATING AND INSERTING PICTURES. CLICK THE EDIT BUTTON ON THE UPPER LEFT SIDE OF THE PAGE AFTER YOU HAVE SIGNED IN.