Team:Washington

From 2011.igem.org

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Current biofuels are made up of compounds that have properties that make them not a perfect substitute for
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gasoline. A much better solution would be to use bacteria to produce alkanes, the main chemical found in gasoline.
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This system would allow for the production of net-carbon neutral gasoline from organisms that can directly or
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indirectly utilize carbon dioxide. One of the goals of our team is to optimize alkane production in ''E. coli'' as
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a model for alkane production in such organisms.
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A previous iGem team(Cambridge 2010) worked with luciferase, a protein from ''Vibrio'' that produces
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<html><meta name="google-site-verification" content="fg3_xZB6BF10NZTT7oSIbF6AmRx0o-b-VZdgok0O3Ok" /></html>
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bioluminescence. This system has been shown to work well in ''E. coli'', but has not been used in brewer's yeast(''Saccharomyces cerevisiae''). We are attempting to port the luciferase system into yeast, both to improve the engineering of yeasst using standardized parts, and to  take steps towards producing beer that glows( because glowing beer would be awesome).
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=<center>'''Make It or Break It: <br/> Diesel Production and Gluten Destruction, the Synthetic Biology Way'''</center>=
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<center>Synthetic biology holds great promise regarding the production of important compounds, and the degradation of harmful ones. This summer, we harnessed the power of synthetic biology to meet the world’s needs for fuel and medicine.</center>
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[[Image:Washington_Fire.jpg|left|320px|borderless|link=https://2011.igem.org/Team:Washington/Alkanes/Background]]
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[[Image:Washington_Bottle.jpg|right|200px|borderless|link=https://2011.igem.org/Team:Washington/Celiacs/Background]]
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[https://2011.igem.org/Team:Washington/Alkanes/Background '''Make It: Diesel Production''']  We constructed a strain of ''Escherichia coli'' that produces a variety of alkanes, the main constituents of diesel fuel, by introducing a pair of genes recently shown to convert fatty acid synthesis intermediates into alkanes. 
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Celiac disease is a genetic disorder in which the human digestive system is unable to properly process gluten, a protein found in wheat. Several attempts to produce an enzyme capable of cleaving gluten have been made, but no viable enzyme has been fully implimented. Our team is attempting to engineer an enzyme capable of both surviving in the digestive system, and be able to still cleave gluten.
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[https://2011.igem.org/Team:Washington/Celiacs/Background '''Break It: Gluten Destruction''']  We identified a protease with gluten-degradation potential, and then reengineered it to have greatly increased gluten-degrading activity, allowing for the breakdown of gluten in the digestive track when taken in pill form.
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[https://2011.igem.org/Team:Washington/Magnetosomes/Background '''iGEM Toolkits''']  To enable next-generation cloning of standard biological parts, we built BioBrick vectors optimized for Gibson assembly and used them to create the Magnetosome Toolkit: a set of 18 genes from an essential operon in magnetotactic bacteria which we are characterizing to create magnetic ''E. coli''.
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Several bacterial species are known to produce magnetosomes, organelles containing small magnetite crystals that allow for orientation to an external magnetic field. The genetic basis of magnetetosomes is not completely understood, but specific genes have been implicated in magnetosome formation. We are putting some of these genes into ''E. coli'' to attempt to show production of magnetosomes, or magnetosome intermediates.  
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[[File:Washington_Spacer.jpg|1px]]
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|[[Image:Washington_team.png|right|frame|Your team picture]]
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[[Image:UW Diesel Front Page.png|300px|link=https://2011.igem.org/Team:Washington/Alkanes/Background]]
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[[File:Washington_Spacer.jpg|20px]]
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[[Image:UW Toolkits Front Page.png|300px|link=https://2011.igem.org/Team:Washington/Magnetosomes/Background]]
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|align="center"|[[Team:Washington | Team Example]]
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[[Image:UW Gluten Front Page.png|300px|link=https://2011.igem.org/Team:Washington/Celiacs/Background]]
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<!--- The Mission, Experiments --->
 
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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<br/>
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!align="center"|[[Team:Washington|Home]]
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!align="center"|[[Team:Washington/Team|Team]]
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[[File:Washington_OSLI.png|frameless|border|150px|link=http://www.osli.ca|Oil Sands Leadership Intiative]]
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!align="center"|[https://igem.org/Team.cgi?year=2011&team_name=Washington Official Team Profile]
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!align="center"|[[Team:Washington/Project|Project]]
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[[File:Washington_UniversitySeal.gif|frameless|border|100px|link=http://www.washington.edu|University of Washington]]
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!align="center"|[[Team:Washington/Parts|Parts Submitted to the Registry]]
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[[File:Washington_Spacer.jpg|25px]]
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!align="center"|[[Team:Washington/Modeling|Modeling]]
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[[File:Washington_ARPA-E_Logo.png|frameless|border|150px|link=http://arpa-e.energy.gov/ProgramsProjects/Electrofuels.aspx|Advanced Research Projects Agency - Energy]]
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!align="center"|[[Team:Washington/Notebook|Notebook]]
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!align="center"|[[Team:Washington/Safety|Safety]]
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[[File:Washington2011_Hhmi_362_72.jpg|link=http://www.hhmi.org/|Howard Hughes Medical Institute]]
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!align="center"|[[Team:Washington/Attributions|Attributions]]
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[[File:Washington2011_NSFlogo.jpg|frameless|border|link=http://www.nsf.gov/|National Science Foundation]]
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[[File:Washington_Anaspec.gif|frameless|border|100px|link=http://www.anaspec.com|Anaspec]]

Latest revision as of 17:01, 2 December 2011


Make It or Break It:
Diesel Production and Gluten Destruction, the Synthetic Biology Way

Synthetic biology holds great promise regarding the production of important compounds, and the degradation of harmful ones. This summer, we harnessed the power of synthetic biology to meet the world’s needs for fuel and medicine.


borderless
borderless

Make It: Diesel Production We constructed a strain of Escherichia coli that produces a variety of alkanes, the main constituents of diesel fuel, by introducing a pair of genes recently shown to convert fatty acid synthesis intermediates into alkanes.

Break It: Gluten Destruction We identified a protease with gluten-degradation potential, and then reengineered it to have greatly increased gluten-degrading activity, allowing for the breakdown of gluten in the digestive track when taken in pill form.

iGEM Toolkits To enable next-generation cloning of standard biological parts, we built BioBrick vectors optimized for Gibson assembly and used them to create the Magnetosome Toolkit: a set of 18 genes from an essential operon in magnetotactic bacteria which we are characterizing to create magnetic E. coli.


Washington Spacer.jpg UW Diesel Front Page.png Washington Spacer.jpg UW Toolkits Front Page.png Washington Spacer.jpg UW Gluten Front Page.png Washington Spacer.jpg



Washington Spacer.jpg Oil Sands Leadership Intiative Washington Spacer.jpg University of Washington Washington Spacer.jpg Advanced Research Projects Agency - Energy Washington Spacer.jpg Howard Hughes Medical Institute Washington Spacer.jpg National Science Foundation Washington Spacer.jpg Anaspec

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