Team:Uppsala-Sweden/Parts

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Submitted parts

  BBa_K592000Codingcph8 (Cph1/EnvZ fusion)
  BBa_K592001CodingCcaS, green light sensor from Synechocystis sp. PCC6803
   BBa_K592002CodingccaR
  BBa_K592003RegulatoryPcpcG2 promoter, ccaR-regulated
   BBa_K592004CodingYF1 blue-light sensor protein
  BBa_K592005CodingFixJ, the response regulator to YF1
  BBa_K592006RegulatoryFixK2 promoter
   BBa_K592008RegulatoryT5-lac Promoter
   BBa_K592009CodingamilCP, blue chromoprotein
   BBa_K592010CodingamilGFP, yellow chromoprotein
   BBa_K592011CodingcjBlue, green chromoprotein
  BBa_K592012CodingeforRed, red chromoprotein
   BBa_K592013CompositeHO1-pcyA, no promoter
  BBa_K592014CompositeHO1-pcyA, low expression, B1001 terminator
  BBa_K592015ReporteramilCP Blue Coding Device
  BBa_K592016CompositeB0034-YF1-B0034-FixJ, blue light sensor and RR with RBS
  BBa_K592017CompositeB0034-ccaS-B0034-ccaR
  BBa_K592018CompositeB0034-cph8
   BBa_K592019ReporterPcpcG2-B0034-amilGFP green light sensor output
  BBa_K592020ReporterPFixK2-lambda C1-B0034-amilCP blue light sensor output
  BBa_K592021ReporterPompC-tetR inverter-B0034-mCherry
  BBa_K592022ReportercjBlue Green Coding Device
  BBa_K592023IntermediateB0032-BFP
  BBa_K592024ReporterB0034-BFP
  BBa_K592025ReporterB0034-amilCP
   BBa_K592026MeasurementJ23101-B0032-BFP
  BBa_K592027MeasurementT5-lac promoter-B0032-BFP
  BBa_K592028MeasurementT5-lac promoter-B0032-YFP
  BBa_K592029MeasurementPcpcG2-B0034-BFP green light sensor output
  BBa_K592030MeasurementPcpcG2-B0034-YFP green light sensor output
  BBa_K592031ReporterJ23101-B0034-amilCP Blue Coding Device
   BBa_K592032MeasurementJ23101-B0032-YFP
   BBa_K592033ReporterPcpcG2-B0034
   BBa_K592100CodingBlue Fluorescent Protein (mTagBFP)
   BBa_K592101CodingYellow Fluorescent Protein (YFP)
   BBa_K592200Plasmid_BackboneLow to medium copy BioBrick standard vector
  BBa_K592201Plasmid_BackboneLow to medium copy Lambda Red recombineering compatible plasmid
   BBa_K592202Plasmid_BackboneLow to medium copy Lambda Red recombineering compatible plasmid

Furthermore, Uppsala-Sweden has submitted 4 intermediate constructs that do not have any obvious function by themselves. However, these constructs contain sequence information that could be valuable to assess the quality of the complete constructs. These constructs are <partinfo>BBa_S04614</partinfo> to <partinfo>BBa_S04617</partinfo>.

Our favorite parts are:

<partinfo>BBa_K592009</partinfo> the amilCP blue chromoprotein, displaying strong color and extremely useful as reporter;

<partinfo>BBa_K592100</partinfo> the Blue Fluorescent Protein (mTagBFP), with its excitation peak at 399 nm and emission peak at 456 nm, a reporter well suited for e.g. flow cytometers with violet laser (e.g. FACSAria II) and DAPI filter (band pass 450/40);

<partinfo>BBa_K592200</partinfo> the "Low to medium copy BioBrick standard vector", specially designed to be useful for Lambda Red recombineering in E.coli, and contains spectinomycin antibiotic resistance marker.

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