Team:Uppsala-Sweden/Parts

From 2011.igem.org

Visit our blog:

http://igem2011uu.blogspot.com

Submitted parts


BBa_K592000	Coding	cph8 (Cph1/EnvZ fusion)
BBa_K592001	Coding	CcaS, green light sensor from Synechocystis sp. PCC6803
BBa_K592002	Coding	ccaR
BBa_K592003	Regulatory	PcpcG2 promoter, ccaR-regulated
BBa_K592004	Coding	YF1 blue-light sensor protein
BBa_K592005	Coding	FixJ, the response regulator to YF1
BBa_K592006	Regulatory	FixK2 promoter
BBa_K592008	Regulatory	T5-lac Promoter
BBa_K592009	Coding	amilCP, blue chromoprotein
BBa_K592010	Coding	amilGFP, yellow chromoprotein
BBa_K592011	Coding	cjBlue, green chromoprotein
BBa_K592012	Coding	eforRed, red chromoprotein
BBa_K592013	Composite	HO1-pcyA, no promoter
BBa_K592014	Composite	HO1-pcyA, low expression, B1001 terminator
BBa_K592015	Reporter	amilCP Blue Coding Device
BBa_K592016	Composite	B0034-YF1-B0034-FixJ, blue light sensor and RR with RBS
BBa_K592017	Composite	B0034-ccaS-B0034-ccaR
BBa_K592018	Composite	B0034-cph8
BBa_K592019	Reporter	PcpcG2-B0034-amilGFP green light sensor output
BBa_K592020	Reporter	PFixK2-lambda C1-B0034-amilCP blue light sensor output
BBa_K592021	Reporter	PompC-tetR inverter-B0034-mCherry
BBa_K592022	Reporter	cjBlue Green Coding Device
BBa_K592023	Intermediate	B0032-BFP
BBa_K592024	Reporter	B0034-BFP
BBa_K592025	Reporter	B0034-amilCP
BBa_K592026	Measurement	J23101-B0032-BFP
BBa_K592027	Measurement	T5-lac promoter-B0032-BFP
BBa_K592028	Measurement	T5-lac promoter-B0032-YFP
BBa_K592029	Measurement	PcpcG2-B0034-BFP green light sensor output
BBa_K592030	Measurement	PcpcG2-B0034-YFP green light sensor output
BBa_K592031	Reporter	J23101-B0034-amilCP Blue Coding Device
BBa_K592032	Measurement	J23101-B0032-YFP
BBa_K592033	Reporter	PcpcG2-B0034
BBa_K592100	Coding	Blue Fluorescent Protein (mTagBFP)
BBa_K592101	Coding	Yellow Fluorescent Protein (YFP)
BBa_K592200	Plasmid_Backbone	Low to medium copy BioBrick standard vector
BBa_K592201	Plasmid_Backbone	Low to medium copy Lambda Red recombineering compatible plasmid
BBa_K592202	Plasmid_Backbone	Low to medium copy Lambda Red recombineering compatible plasmid

Furthermore, Uppsala-Sweden has submitted 4 intermediate constructs that do not have any obvious function by themselves. However, these constructs contain sequence information that could be valuable to assess the quality of the complete constructs. These constructs are <partinfo>BBa_S04614</partinfo> to <partinfo>BBa_S04617</partinfo>.

Our favorite parts are:

<partinfo>BBa_K592009</partinfo> the amilCP blue chromoprotein, displaying strong color and extremely useful as reporter;

<partinfo>BBa_K592100</partinfo> the Blue Fluorescent Protein (mTagBFP), with its excitation peak at 399 nm and emission peak at 456 nm, a reporter well suited for e.g. flow cytometers with violet laser (e.g. FACSAria II) and DAPI filter (band pass 450/40);

<partinfo>BBa_K592200</partinfo> the "Low to medium copy BioBrick standard vector", specially designed to be useful for Lambda Red recombineering in E.coli, and contains spectinomycin antibiotic resistance marker.

.