Team:UT-Tokyo/Project/Results

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Assembly parts

Digest

Ligation

colony PCR

Transformation

Making Competent E. coli cell

Transformation of E. coli

Preparation

iGEM parts / ligation products
SOC or LB (No antibiotic)　500μL
TE　15μL
plates
ice box
heat block(42℃)
competent cells

→ always on ice! Melt on ice! Mix DNA as soon as cells melt!

Protocol

to thaw out igem parts

  1. With a pipette tip, punch a hole in the foil
  2. Add 15uL of TE (MilliQ),and pipetting
  3. Pipette 1uL of the resuspended DNA Transformation into your desired competent cells
  4. Hold on ice for 30 mins
  5. Heat shock at 42°C for 45 seconds (and on ice after it)
  6. Add 300uL of LBborth in each epp
  7. Wait for 10 mins
  8. Hold at 37℃ for 30 mins
  9. Plate out
  10. Incubate at 37°C

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