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From 2011.igem.org

Revision as of 11:20, 24 September 2011 (view source) Akira (Talk | contribs) m ← Older edit		Revision as of 11:23, 24 September 2011 (view source) Akira (Talk | contribs) m Newer edit →
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	</html>		</html>
-	==Assembly parts==	+	==Assey 1==
-	===Digest===	+
-	===Ligation===
-
-	===colony PCR===
-
-	==Transformation==
-	===Making Competent E. coli cell===
-
-	===Transformation of E. coli===
	<div id="top-panel">		<div id="top-panel">
-	====Preparation====	+	====Test====
-		+
-	:iGEM parts / ligation products	+
-	:SOC or LB (No antibiotic)　500μL	+
-	:TE　15μL	+
-	:plates	+
-	:ice box	+
-	:heat block(42℃)	+
-	:competent cells	+
-	→ always on ice! Melt on ice! Mix DNA as soon as cells melt!	+
-		+
-	====Protocol====	+
-	to thaw out igem parts
-	:#With a pipette tip, punch a hole in the foil
-	:#Add 15uL of TE (MilliQ),and pipetting
-	:#Pipette 1uL of the resuspended DNA Transformation into your desired competent cells
-	:#Hold on ice for 30 mins
-	:#Heat shock at 42°C for 45 seconds (and on ice after it)
-	:#Add 300uL of LBborth in each epp
-	:#Wait for 10 mins
-	:#Hold at 37℃ for 30 mins
-	:#Plate out
-	:#Incubate at 37°C
	</div>		</div>
	{{:Team:UT-Tokyo/Templates/EndContent}}		{{:Team:UT-Tokyo/Templates/EndContent}}

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Revision as of 11:23, 24 September 2011