Team:UNITS Trieste/Project

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<h3>INTERKINGDOM SOCIAL NETWORK</h3>
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<p>An important challenge in the near future will be the optimization of bioreactors for the production of complex molecules. The aim of our research project is to combine different cell systems commonly used in biosynthesis through synthetic biology. To improve this system we want to use cells from different kingdoms because we believe that different cell types could cooperate and better produce complex molecules. The innovation and challenge will be to obtain a stable community of cells from different kingdoms and establish mutualism among them. This interdependence will be obtained through a metabolic and signaling pathways in which the survival and/or growth depends from the other cell types. The project is based on a three-element system: two different bacterial strains and one eukaryotic cell type that communicate through quorum sensing (QS) signal molecules. In order to achieve the goal in constructing this synthetic community, both the bacterial cells and the eukaryotic cell will be engineered with a genetic circuit under the regulation of the N-acyl homoserine lactone (AHL) QS signals.<br/>
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More specifically, we will engineer both bacterial strains to produce the enzyme cellobiosidase, in order to convert extracellular cellobiose into glucose, while the eukaryotic cell will be engineered to produce a soluble form of beta-lactamase.<br/>
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This set up will ensure interdependence among the three cell types; all cells will benefit from the free available glucose and the two bacteria will survive in an ampicillin-containing culture medium. The mutalism between the two different bacterial strains will occur thanks to a synthetic network based on the two different AHL QS signals, namely <b>3-oxo-C8-AHL</b> and <b>3-oxo-C12-AHL</b>.<br/>
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The inter-kingdom mutualism will be guaranteed by an eukaryotic trans-activator sensible to the AHL QS mediator <b>3-oxo-C8-AHL</b>.<br/>
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Importantly, this genetic circuit will be designed in such a way so that it can be adapted to different bacterial species and eukaryotic cell types.</p>
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace. 
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{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:UNITS_Trieste_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|[[Image:UNITS_Trieste_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:UNITS_Trieste | Team Example]]
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:UNITS_Trieste|Home]]
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!align="center"|[[Team:UNITS_Trieste/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=UNITS_Trieste Official Team Profile]
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!align="center"|[[Team:UNITS_Trieste/Project|Project]]
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!align="center"|[[Team:UNITS_Trieste/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:UNITS_Trieste/Modeling|Modeling]]
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!align="center"|[[Team:UNITS_Trieste/Notebook|Notebook]]
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!align="center"|[[Team:UNITS_Trieste/Safety|Safety]]
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!align="center"|[[Team:UNITS_Trieste/Attributions|Attributions]]
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Revision as of 13:32, 15 July 2011

INTERKINGDOM SOCIAL NETWORK

An important challenge in the near future will be the optimization of bioreactors for the production of complex molecules. The aim of our research project is to combine different cell systems commonly used in biosynthesis through synthetic biology. To improve this system we want to use cells from different kingdoms because we believe that different cell types could cooperate and better produce complex molecules. The innovation and challenge will be to obtain a stable community of cells from different kingdoms and establish mutualism among them. This interdependence will be obtained through a metabolic and signaling pathways in which the survival and/or growth depends from the other cell types. The project is based on a three-element system: two different bacterial strains and one eukaryotic cell type that communicate through quorum sensing (QS) signal molecules. In order to achieve the goal in constructing this synthetic community, both the bacterial cells and the eukaryotic cell will be engineered with a genetic circuit under the regulation of the N-acyl homoserine lactone (AHL) QS signals.
More specifically, we will engineer both bacterial strains to produce the enzyme cellobiosidase, in order to convert extracellular cellobiose into glucose, while the eukaryotic cell will be engineered to produce a soluble form of beta-lactamase.
This set up will ensure interdependence among the three cell types; all cells will benefit from the free available glucose and the two bacteria will survive in an ampicillin-containing culture medium. The mutalism between the two different bacterial strains will occur thanks to a synthetic network based on the two different AHL QS signals, namely 3-oxo-C8-AHL and 3-oxo-C12-AHL.
The inter-kingdom mutualism will be guaranteed by an eukaryotic trans-activator sensible to the AHL QS mediator 3-oxo-C8-AHL.
Importantly, this genetic circuit will be designed in such a way so that it can be adapted to different bacterial species and eukaryotic cell types.