Team:UNITS Trieste/Acknowledgements
From 2011.igem.org
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+ | <span>Edinburgh IGEM team</span> | ||
+ | <div class="figure"><a href="https://2011.igem.org/Team:Edinburgh"><img style="padding-top:55px" width="200" src="https://static.igem.org/mediawiki/2011/d/d9/Edinburgh_home_logo.png"/></a></div> | ||
+ | <p>During the theoretical work on Synbiome we decided to use a β-glucosidase to provide our Interkingdom Consortium with a usable carbon source. We asked Chris French to send us the part BBa_K392008, made by team Osaka in 2010 and encoding a β-glucosidase from the bacterium Cellulomonas fimi. Firstly, we tested the part and it worked, but the DNA sequencing we decided to make during the generation of our parts BBa_K553005 and BBa_K553006 underlined the presence of a frameshift at the beginning of the coding sequence, introducing a stop codon. We shared this information with team Edinburgh and together we elaborated a solution to this strange problem (more details can be found on the cooperation page of team Edinburgh’s website). They were also very kind to provide us with a protocol of an assay that can be used to test the activity of this β-glucosidase using 4-methylumbelliferyl-β-D-glucuronide.</p></div> | ||
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<div id="cooperations" class="slide4 full-slides"> | <div id="cooperations" class="slide4 full-slides"> | ||
<h1>COOPERATIONS</h1> | <h1>COOPERATIONS</h1> | ||
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<div class="cooperations" style="background-image:url(https://static.igem.org/mediawiki/2011/b/b2/Units-bkgR.png)" > | <div class="cooperations" style="background-image:url(https://static.igem.org/mediawiki/2011/b/b2/Units-bkgR.png)" > |
Revision as of 08:46, 6 October 2011
During the theoretical work on Synbiome we decided to use a β-glucosidase to provide our Interkingdom Consortium with a usable carbon source. We asked Chris French to send us the part BBa_K392008, made by team Osaka in 2010 and encoding a β-glucosidase from the bacterium Cellulomonas fimi. Firstly, we tested the part and it worked, but the DNA sequencing we decided to make during the generation of our parts BBa_K553005 and BBa_K553006 underlined the presence of a frameshift at the beginning of the coding sequence, introducing a stop codon. We shared this information with team Edinburgh and together we elaborated a solution to this strange problem (more details can be found on the cooperation page of team Edinburgh’s website). They were also very kind to provide us with a protocol of an assay that can be used to test the activity of this β-glucosidase using 4-methylumbelliferyl-β-D-glucuronide.