Team:UNITS Trieste

From 2011.igem.org

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<h1>NOTEBOOK</h1>
<h1>NOTEBOOK</h1>
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<b>1st Week</b> - Our lab work starts off with the arrival of the two plasmids pcD/p65NTraR and pSEAP-(tra box)(1-7) (Neddermann P. et al., 2003) and of the commercial vector pIRES2-EGFP
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<b>4th Week</b> - We finally obtained and amplified pIRES2-EGFP without CMVie and without the gene Rac2, that we discovered was cloned inside the MCS.
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<a href="https://2011.igem.org/Team:UNITS_Trieste/Notebook#1st_Week"> Read more...</a><br/><br/>
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<a href="https://2011.igem.org/Team:UNITS_Trieste/Notebook#4th_Week"> Read more...</a><br/><br/>
<b>2nd Week</b> - The oligos we requested the earlier week arrived, and we proceeded with the annealing protocol for
<b>2nd Week</b> - The oligos we requested the earlier week arrived, and we proceeded with the annealing protocol for
the two pairs of complementary strands
the two pairs of complementary strands
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<a href="https://2011.igem.org/Team:UNITS_Trieste/Notebook#2nd_Week"> Read more...</a>
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<b>5th Week</b> - We tested the activity of pSEAP after co-transfection with p65-TraR into HeLa cells.
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<a href="https://2011.igem.org/Team:UNITS_Trieste/Notebook#5th_Week"> Read more...</a>
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Revision as of 20:55, 8 August 2011

NEWS ON TWITTER

    NOTEBOOK

    4th Week - We finally obtained and amplified pIRES2-EGFP without CMVie and without the gene Rac2, that we discovered was cloned inside the MCS. Read more...

    2nd Week - The oligos we requested the earlier week arrived, and we proceeded with the annealing protocol for the two pairs of complementary strands 5th Week - We tested the activity of pSEAP after co-transfection with p65-TraR into HeLa cells. Read more...

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