Team:UNICAMP-EMSE Brazil/Notebook/19 September 2011

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(Difference between revisions)
(Created page with "{{Template:UNICAMP-EMSE Brazil_Notebook}} <div style="text-align: left;"> ===Ligations:=== => IL-10 (~550bp) and pSB1C3 (~2000bp) (ratio for ligation 3:1)<br> 3.8 ul - H2...")
(→Ligations:)
 
Line 29: Line 29:
15      ul  - digested HlyD (~180 ng )<br>
15      ul  - digested HlyD (~180 ng )<br>
2.6      ul  - digested vector (~60 ng)<br>
2.6      ul  - digested vector (~60 ng)<br>
 +
2.5      ul  - 10X Ligation Buffer<br>
 +
1        ul  - T4 Ligase<br>
 +
_________________________________
 +
25      ul  => TOTAL<br>
 +
 +
 +
=> QseB/C (~2100bp) and pSB1C3 (~2000bp) (ratio for ligation 1:1)<br>
 +
 +
10.1 ul  - H20<br>
 +
4.3  ul  - digested QseB-QseC (~60 ng )<br>
 +
2.6  ul  - digested vector [pSB] (~60 ng)<br>
 +
2      ul  - 10X Ligation Buffer<br>
 +
1      ul  - T4 Ligase<br>
 +
_________________________________
 +
20    ul  => TOTAL<br>
 +
 +
 +
=> HlyB (~2200bp) and pSB1C3 (~2000bp) (ratio for ligation 1:1)<br>
 +
 +
10.9    ul  - H20<br>
 +
3.5      ul  - digested HlyB (~60 ng )<br>
 +
2.6      ul  - digested vector [pSB] (~60 ng)<br>
 +
2        ul  - 10X Ligation Buffer<br>
 +
1        ul  - T4 Ligase<br>
 +
_________________________________
 +
20    ul  => TOTAL<br>
 +
 +
 +
=> TolC (1580bp) and pSB1C3 (~2000bp) (ratio for ligation 2:1)<br>
 +
 +
20      ul  - digested TolC (~80 ng )<br>
 +
1.5      ul  - digested vector [pSB] (~40 ng)<br>
 +
2.5      ul  - 10X Ligation Buffer<br>
 +
1        ul  - T4 Ligase<br>
 +
_________________________________
 +
25      ul  => TOTAL<br>
 +
 +
 +
=> SoxS (~103bp) and pSB1C3 (~2000bp) (ratio for ligation 3:1)<br>
 +
 +
0.3      ul  - H20<br>
 +
20      ul  - digested SoxS (~88 ng )<br>
 +
1.2      ul  - digested vector [pSB] (~29 ng)<br>
2.5      ul  - 10X Ligation Buffer<br>
2.5      ul  - 10X Ligation Buffer<br>
1        ul  - T4 Ligase<br>
1        ul  - T4 Ligase<br>
_________________________________
_________________________________
25      ul  => TOTAL<br>
25      ul  => TOTAL<br>

Latest revision as of 02:11, 29 September 2011

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Notebook

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May
MTWTFSS
            [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/1_May_2011&action=edit 1]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/2_May_2011&action=edit 2] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_May_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_May_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_May_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_May_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/7_May_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/8_May_2011&action=edit 8]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_May_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_May_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_May_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_May_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/13_May_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_May_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_May_2011&action=edit 15]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/16_May_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_May_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_May_2011&action=edit 18] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_May_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_May_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/21_May_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/22_May_2011&action=edit 22]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_May_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_May_2011&action=edit 24] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_May_2011 25] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_May_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_May_2011 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_May_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/29_May_2011&action=edit 29]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/30_May_2011 30] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/31_May_2011 31]
June
MTWTFSS
    [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/1_June_2011 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_June_2011 2] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/3_June_2011 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_June_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_June_2011&action=edit 5]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/6_June_2011 6] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/7_June_2011 7] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/8_June_2011 8] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/9_June_2011 9] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/10_June_2011 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_June_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_June_2011&action=edit 12]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/13_June_2011 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_June_2011&action=edit 14] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/15_June_2011 15] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/16_June_2011 16] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/17_June_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_June_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/19_June_2011&action=edit 19]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/20_June_2011 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_June_2011 21] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_June_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_June_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_June_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/25_June_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/26_June_2011&action=edit 26]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/27_June_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_June_2011&action=edit 28] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/29_June_2011 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_June_2011&action=edit 30]
July
MTWTFSS
        [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/1_July_2011&action=edit 1] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/2_July_2011&action=edit 2] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/3_July_2011 3]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_July_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_July_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_July_2011&action=edit 6] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/7_July_2011 7] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/8_July_2011 8] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_July_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_July_2011&action=edit 10]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/11_July_2011 11] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/12_July_2011 12] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/13_July_2011 13] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/14_July_2011 14] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/15_July_2011 15] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/16_July_2011 16] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_July_2011&action=edit 17]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/18_July_2011 18] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_July_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_July_2011&action=edit 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_July_2011 21] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_July_2011 22] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_July_2011&action=edit 23] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_July_2011&action=edit 24]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_July_2011 25] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_July_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_July_2011 27] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/28_July_2011 28] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/29_July_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_July_2011&action=edit 30] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/31_July_2011&action=edit 31]
August
MTWTFSS
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/1_August_2011 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_August_2011 2] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_August_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_August_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_August_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_August_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/7_August_2011&action=edit 7]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/8_August_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_August_2011&action=edit 9] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_August_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_August_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_August_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/13_August_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_August_2011&action=edit 14]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_August_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/16_August_2011&action=edit 16] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_August_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_August_2011&action=edit 18] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_August_2011 19] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/20_August_2011 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_August_2011 21]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_August_2011 22] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/23_August_2011 23] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/24_August_2011 24] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_August_2011 25] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_August_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_August_2011 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_August_2011&action=edit 28]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/29_August_2011 29] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/30_August_2011 30] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/31_August_2011 31]
September
MTWTFSS
      [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/1_September_2011 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_September_2011 2] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_September_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_September_2011&action=edit 4]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/5_September_2011 5] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/6_September_2011 6] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/7_September_2011 7] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/8_September_2011 8] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/9_September_2011 9] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/10_September_2011 10] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/11_September_2011 11]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/12_September_2011 12] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/13_September_2011 13] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/14_September_2011 14] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_September_2011&action=edit 15] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/16_September_2011 16] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/17_September_2011 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_September_2011&action=edit 18]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/19_September_2011 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_September_2011&action=edit 20] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/21_September_2011 21] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/22_September_2011 22] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/23_September_2011 23] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/24_September_2011 24] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/25_September_2011 25]
[http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/26_September_2011 26] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/27_September_2011 27] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/28_September_2011 28] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/29_September_2011 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_September_2011&action=edit 30]
October
MTWTFSS
          [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/1_October_2011&action=edit 1] [http://2011.igem.org/Team:UNICAMP-EMSE_Brazil/Notebook/2_October_2011 2]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/3_October_2011&action=edit 3] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/4_October_2011&action=edit 4] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/5_October_2011&action=edit 5] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/6_October_2011&action=edit 6] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/7_October_2011&action=edit 7] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/8_October_2011&action=edit 8] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/9_October_2011&action=edit 9]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/10_October_2011&action=edit 10] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/11_October_2011&action=edit 11] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/12_October_2011&action=edit 12] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/13_October_2011&action=edit 13] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/14_October_2011&action=edit 14] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/15_October_2011&action=edit 15] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/16_October_2011&action=edit 16]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/17_October_2011&action=edit 17] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/18_October_2011&action=edit 18] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/19_October_2011&action=edit 19] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/20_October_2011&action=edit 20] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/21_October_2011&action=edit 21] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/22_October_2011&action=edit 22] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/23_October_2011&action=edit 23]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/24_October_2011&action=edit 24] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/25_October_2011&action=edit 25] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/26_October_2011&action=edit 26] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/27_October_2011&action=edit 27] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/28_October_2011&action=edit 28] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/29_October_2011&action=edit 29] [http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/30_October_2011&action=edit 30]
[http://2011.igem.org/wiki/index.php?title=Team:UNICAMP-EMSE_Brazil/Notebook/31_October_2011&action=edit 31]


Ligations:

=> IL-10 (~550bp) and pSB1C3 (~2000bp) (ratio for ligation 3:1)

3.8 ul - H20
20 ul - digested IL-10 (~150 ng )
2.2 ul - digested vector [pSB] (~50 ng)
3 ul - 10X Ligation Buffer
1 ul - T4 Ligase
_________________________________ 30 ul => TOTAL


=> IL-12 (~1660bp) and pSB1C3 (~2000bp) (ratio for ligation 3:1)

3.9 ul - H20
15 ul - digested IL-12 (~180 ng )
2.6 ul - digested vector [pSB] (~60 ng)
2.5 ul - 10X Ligation Buffer
1 ul - T4 Ligase
_________________________________ 25 ul => TOTAL

=> HlyD (~1500bp) and pSB1C3 (~2000bp) (ratio for ligation 3:1)

3.9 ul - H20
15 ul - digested HlyD (~180 ng )
2.6 ul - digested vector (~60 ng)
2.5 ul - 10X Ligation Buffer
1 ul - T4 Ligase
_________________________________ 25 ul => TOTAL


=> QseB/C (~2100bp) and pSB1C3 (~2000bp) (ratio for ligation 1:1)

10.1 ul - H20
4.3 ul - digested QseB-QseC (~60 ng )
2.6 ul - digested vector [pSB] (~60 ng)
2 ul - 10X Ligation Buffer
1 ul - T4 Ligase
_________________________________ 20 ul => TOTAL


=> HlyB (~2200bp) and pSB1C3 (~2000bp) (ratio for ligation 1:1)

10.9 ul - H20
3.5 ul - digested HlyB (~60 ng )
2.6 ul - digested vector [pSB] (~60 ng)
2 ul - 10X Ligation Buffer
1 ul - T4 Ligase
_________________________________ 20 ul => TOTAL


=> TolC (1580bp) and pSB1C3 (~2000bp) (ratio for ligation 2:1)

20 ul - digested TolC (~80 ng )
1.5 ul - digested vector [pSB] (~40 ng)
2.5 ul - 10X Ligation Buffer
1 ul - T4 Ligase
_________________________________ 25 ul => TOTAL


=> SoxS (~103bp) and pSB1C3 (~2000bp) (ratio for ligation 3:1)

0.3 ul - H20
20 ul - digested SoxS (~88 ng )
1.2 ul - digested vector [pSB] (~29 ng)
2.5 ul - 10X Ligation Buffer
1 ul - T4 Ligase
_________________________________

25 ul => TOTAL
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