Team:UCSF/MGFP5
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+ | <h3red>MgFP5</h3red> <p> | ||
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+ | <regulartext>Mgfp-5 is an adhesive protein present in the foot of mussels. It is known to be an | ||
+ | important part of what makes mussels stick on surfaces. We hoped to express | ||
+ | mgfp-5 on the surface of S. cerevisiae to enhance its adherence to materials such as | ||
+ | polystyrene or treated plastics and promote adherence to other yeast cells. | ||
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+ | We discovered that it already was a part in the Parts Registry, but only | ||
+ | characterized in E. coli. The previous team expressed it in E. coli and ran a protein | ||
+ | quantitative assay, the results of which can be found <a href=http://partsregistry.org/Part:BBa_K197018>here</a>. We decided that we’d take a different approach in assaying the | ||
+ | results, to observe what mgfp-5-expressing yeast grew like under the microscope | ||
+ | and how it adhered in flowing conditions. | ||
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+ | Our results show conclusive evidence that mgfp-5-expressing yeast “cluster”, form | ||
+ | clumps of cells that grow on and around on another, more often and to a larger | ||
+ | degree than non-mgfp-5-expressing yeast. We hope that this could be used to create | ||
+ | a biomaterial or a bioadhesive that could be natural and biodegradable.</regulartext> | ||
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+ | <regulartext1> <b><br><BR><BR><BR><BR><br><BR><BR><BR><BR><BR><bR><BR><BR><BR><BR>Figure 1:</b> Control, non-induced mgfp- | ||
+ | 5 yeast. Dispersed, no major | ||
+ | clustering. </regulartext1> | ||
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+ | <img align="left" style="margin-bottom:0px; width: 300px; margin-top:0px; padding:0;" src="http://dl.dropbox.com/u/24404809/MgfpGal1.jpg"> | ||
+ | <BR> | ||
+ | <regulartext1> <b><br><BR><BR><BR><BR><br><BR><BR><BR><BR><bR><BR><BR><BR><BR>Figure 2:</b> Experiment, induced mgfp- | ||
+ | 5 yeast. 3-D cluster of yeast cells | ||
+ | growing around and on top of other | ||
+ | cells. </regulartext1> | ||
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Latest revision as of 02:19, 29 September 2011
Figure 1: Control, non-induced mgfp- 5 yeast. Dispersed, no major clustering.
Figure 2: Experiment, induced mgfp- 5 yeast. 3-D cluster of yeast cells growing around and on top of other cells.