Team:UCSF/Data2

From 2011.igem.org

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     <a href="http://partsregistry.org/Part:BBa_K644000">Cadherin Parts Registry</a>  - Extracellular Domain of E-Cadherin BBa_K644000: when expressed on the surface of yeast cells, the extracellular domain of E-cadherin caused the yeast cells to stick together and form large clumps, visible by microscopy<p><regulartext>
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     <a href="http://partsregistry.org/Part:BBa_K644000">Cadherin Parts Registry</a>  - Extracellular Domain of E-Cadherin BBa_K644000: when expressed on the surface of yeast cells, the extracellular domain of E-cadherin caused the yeast cells to stick together and form large clumps, visible by microscopy. The addition of calcium to the media caused larger clumps to form and these could be disrupted with the addition of EDTA. <p><regulartext>
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     <a href="http://partsregistry.org/Part:BBa_K644001">Hwp1 Parts Registry</a>  - Insulated Vector, BBa_XXX00: un-induced leaky expression is blocked by transcriptional terminators <p>
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     <a href="http://partsregistry.org/Part:BBa_K644001">Hwp1 Parts Registry</a>  - Hwp1 BBa_K644001: when expressed on the surface of yeast  <p>
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    <regulartext>Main Page - BAR Bad Odor Receptor, BBa_000XX: yellow fluorescent protein-tagged BAR (BBa_X00XX) shows that BAR is produced in E. coli
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<h3red>Data For Pre-Existing Parts</h3red> <p>
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    <a href="http://partsregistry.org/Part:BBa_K197018:Experience#Applications_of_BBa_K197018">Experience - Mgfp-5</a>  - Mussel Foot Protein Mgfp-5 BBa_K197018 (Berkeley iGEM 2009): when expressed on the surface of yeast cells, mgfp5 caused the cells to stick together and form small clumps. <p><regulartext>
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Revision as of 23:16, 28 September 2011

introduction
the team
our project
parts
requisites
attributions
extras

How Our System Works


All of the parts that we created this summer are designed to be fusion proteins with Aga2, a yeast protein involved in mating.




Interaction of Aga1 with Aga2-fused protein of interest leads to Yeast Cell Surface Display









Interaction of Two Cells via Yeast Cell Surface Display

Data For Our Favorite New Parts

Cadherin Parts Registry - Extracellular Domain of E-Cadherin BBa_K644000: when expressed on the surface of yeast cells, the extracellular domain of E-cadherin caused the yeast cells to stick together and form large clumps, visible by microscopy. The addition of calcium to the media caused larger clumps to form and these could be disrupted with the addition of EDTA.

Hwp1 Parts Registry - Hwp1 BBa_K644001: when expressed on the surface of yeast


Data For Pre-Existing Parts

Experience - Mgfp-5 - Mussel Foot Protein Mgfp-5 BBa_K197018 (Berkeley iGEM 2009): when expressed on the surface of yeast cells, mgfp5 caused the cells to stick together and form small clumps.

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