Team:Tokyo Metropolitan/Project/Killing
From 2011.igem.org
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Bacteriophage have cell lysis cassette Holin-Endolysin. Holin forms hole on outer membrane of host cell.</td></tr><tr valign="top"><td width="400">Endolysin is degradation peptide glycan in intermediate membrane of host cell.</td><td><img src="https://static.igem.org/mediawiki/2011/f/f1/Tokyo_Metropolitan_Lysis.jpg" width="400"><br><div align="center">Figure2 Bacteriophage Lysis Casstte</div></td></tr></table> | Bacteriophage have cell lysis cassette Holin-Endolysin. Holin forms hole on outer membrane of host cell.</td></tr><tr valign="top"><td width="400">Endolysin is degradation peptide glycan in intermediate membrane of host cell.</td><td><img src="https://static.igem.org/mediawiki/2011/f/f1/Tokyo_Metropolitan_Lysis.jpg" width="400"><br><div align="center">Figure2 Bacteriophage Lysis Casstte</div></td></tr></table> | ||
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Ptet-TetR system is appropriate Anti-killer Device. In BeE.coli, TetR is expressed constitutively and repressed expression of killing gene. But killing gene has sent to other bacteria which doesn’t have tetR. Then conjugated bacteria cell lysis. In addition, from point of view of biosafety, to add aTc induce BeE.coli to cell lysis.</td><td width="400"><img src="https://static.igem.org/mediawiki/2011/e/ee/Tokyo_Metropolitan_Anti-killer.png" width="398"><br> | Ptet-TetR system is appropriate Anti-killer Device. In BeE.coli, TetR is expressed constitutively and repressed expression of killing gene. But killing gene has sent to other bacteria which doesn’t have tetR. Then conjugated bacteria cell lysis. In addition, from point of view of biosafety, to add aTc induce BeE.coli to cell lysis.</td><td width="400"><img src="https://static.igem.org/mediawiki/2011/e/ee/Tokyo_Metropolitan_Anti-killer.png" width="398"><br> | ||
<div align="center">Figure3: TetR is transcription regulator</div></td></tr></table> | <div align="center">Figure3: TetR is transcription regulator</div></td></tr></table> | ||
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<tr><td colspan="2"><img src="https://static.igem.org/mediawiki/2011/8/84/Tokyo_Metropolitan_Killing1.png" width="800"></td></tr> | <tr><td colspan="2"><img src="https://static.igem.org/mediawiki/2011/8/84/Tokyo_Metropolitan_Killing1.png" width="800"></td></tr> |
Revision as of 17:30, 4 October 2011
Killing Device
Concept When bee attack enemy, they sting needle and inject toxin. In Japan, the most cause of death from animal is attacted from bee (especially wasp).Bee is awful animal but taking a hint from it, we propose new system for killing bacteria using conjugation. | ||||
Conjugation E.coli has sex. “Male” E.coli (having conjugative plasmid) mate other “Female“ E.coli(not having conjugative plasmid). Male E.coli transfer conjugative plasmid to Female E.coli. Then, after conjugation, Female E.coli gets conjugative plasmid and change sex “male”. Conjugation is useful system for killing bacteria. We plan to integrate killing gene (below-mentioned) into the conjugative plasmid and induce this plasmid into E.coli. Then E.coli conjugate other E.coli and send killing gene which induce E.coli to cell lysis when it expressed. | ||||
Cell Lysis
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Anti-killer device
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