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Team:Tokyo Metropolitan/Notebook/S15
From 2011.igem.org
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Transformed P0140 instead of P0440. After, incubated at 37 deg C. | Transformed P0140 instead of P0440. After, incubated at 37 deg C. | ||
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| + | -Colony PCR | ||
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| + | Samples which transformed one day ago | ||
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| + | -Electrophoresis | ||
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| + | Above samples | ||
Latest revision as of 13:32, 5 October 2011
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-Targeting-
-Plasmid extraction and DNA refining
Extracted plasmids for targetingDevice and P0440.
Results
Targeting
75 ng/ul
P0440
20 ng/ul
-Electrophoresis
Checked for P0440, Targeting and P0440.
Results
Checked to cut for P0440 by treat restriction enzyme but length was 1000 bp (too long).
-Transformation
Transformed P0140 instead of P0440. After, incubated at 37 deg C.
-Visualization-
-Colony PCR
Samples which transformed one day ago
-Electrophoresis
Above samples
