Latest revision as of 13:29, 5 October 2011

-Visualization-

@confirmation of transformation　(yesterday)

@restriction enzyme to pSB1C3(backbone for parts) (EcoRI, SpeⅠ)

@electrophore pSB1C3, O+G, total 4 samples.

@restriction enzyme to pSB1C3 (EcoRI, SpeⅠ)

@ligation OG+O ①

@transformation OG+O ①

@ligation OG+O ②

@transformation OG+O ②

-Plasmid extraction

Promoter and suppressed expression system

-DNA refining

Above samples

-Digestion

Above samples

-Electrophoresis

Above samples

-Digestion

Promoter: Spe and Pst

Suppressed expression system: Xba and Pst

@electrophoresis J23100-K317037, K283030, J22005
J23100-K317037, K283030 find binds.
J22005 not find bind.

@gel extraction and confirmation binds by electrophoresis J23100-K317037, K283030

@ligation J23100-K317037 and K283030

@transformation J23100-K317037-K283030

@@ Line 22: / Line 22: @@
 @transformation OG+O ②
+-----------------------------------------------------------------------
+-Plasmid extraction
+Promoter and suppressed expression system
+-DNA refining
+Above samples
+-Digestion
+Above samples
+-Electrophoresis
+Above samples
+-Digestion
+Promoter: Spe and Pst
+Suppressed expression system: Xba and Pst
+= -Targetting- =
+@electrophoresis J23100-K317037, K283030, J22005<Br>
+J23100-K317037, K283030 find binds.<Br>
+J22005 not find bind.<Br>
+@gel extraction and confirmation binds by electrophoresis
+J23100-K317037, K283030
+@ligation J23100-K317037 and K283030
+@transformation J23100-K317037-K283030