-Targeting-

-Electrophoresis Checked to treat restriction enzyme for J04450 and J23100-P0440. Cut only J23100-P0440.

-Plasmid extraction and DNA refining

Extracted plasmids and refined DNA for J23100-K317037, J23100-P0440, K283030 and J22005.

Results (concentration = ng/ul)

J2310-K317037 (Jack) was 60

J23100-P0440 (Jamper) was 120

K283030 (Kasuga) was 155

J22005 (Jin) was 183

-Treat restriction enzyme

Treated restriction enzyme with E and P for J04450. After, incubated at 37 deg C for 5 hours.

-Electrophoresis

Checked to treat restriction enzyme for J04450. Results was a failure.

-Treat restriction enzyme

Jack -> S and P

Jamper -> E and P

Kasuga -> X and P

Jin -> Nhe

had been incubated at 37 deg C after treat restriction enzyme with preceding enzymes.