-Targetting-

@electrophoresis
J23100-K317037, J23100-P0440, K283030, J22005, J04450
All but J04450 confirmationed cut by restriction enzyme.

@gel extraction and DNA refine All but J04450.
In this phase, J23199-P0440 and J22005 were failure.

@preculture J23100-P0440 and J22005

@restriction enzyme J04450 (EcoRⅠ, Pst)

@ligation J23100-K317037 and K283030
for control, self ligation J23100-K317037

@transformation ligation product