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Team:Tokyo Metropolitan/Notebook/S08
From 2011.igem.org
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| + | @preculture from transformed 5th Aug. | ||
Latest revision as of 13:57, 5 October 2011
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-Speeding Up-
Purification of HNS(wt)and electrophoresis and purification of pSB1C3. Although both concentrations were not enough, try to ligate and transformation HNS(wt) and pSB1C3.
-Visualization-
-Colony PCR
Samples which transformed one day ago.
-Electrophoresis
Above samples
-Preculture
Promoter
Suppressed expression system
RFP
@plasmid extraction, DNA refine, DNA densitometry, restriction emzyme(EcoRⅠ, SpeⅠ), electrophoresis OriTr-GFP
@preculture from transformed 5th Aug.
