Team:Tokyo Metropolitan/Notebook/S07

From 2011.igem.org

(Difference between revisions)
(-Speeding Up-)
 
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@preculture<Br>
@preculture<Br>
J23100-K317037 and J23100-P0440 which find binds.
J23100-K317037 and J23100-P0440 which find binds.
 +
 +
 +
= -Visualization- =
 +
-Ligation
 +
 +
Promoter - suppressed expression system

Latest revision as of 13:22, 5 October 2011

-Speeding Up-

Extracted plasmid from E.coli transformated BBa_J04450,

Refined by phenol chloroform mixture and ethanol precipitation,

Added restriction enzyme,

HNS(wt)→X,S

BBa_J04450→X,S


-Targetting-

@plasmid extraction and DNA refine J23100-P0440

@colony PCR J23100-K317037 and J23100-P0440

@electrophoresis
J23100-K317037: find binds in 29 and 31
J23100-P0440: find binds almost all

@preculture
J23100-K317037 and J23100-P0440 which find binds.


-Visualization-

-Ligation

Promoter - suppressed expression system