Team:Tokyo Metropolitan/Notebook/S02

From 2011.igem.org

(Difference between revisions)
(→-Visualization-)
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@transformation OriTr+GFP and OriT, but failure
@transformation OriTr+GFP and OriT, but failure
 +
 +
 +
= -Targeting- =
 +
 +
-Plasmid extraction
 +
 +
Used QlaprepSpinMiniprepkit
 +
 +
 +
Results
 +
 +
J23100
 +
 +
Jaiko 123
 +
 +
Jian 82
 +
 +
 +
P0440
 +
 +
Pisuke 158
 +
 +
Pichiku 155
 +
 +
(ng/ul)
 +
 +
 +
-Electrophoresis
 +
 +
Checked for K317037 and J23100
 +
 +
 +
Results
 +
 +
K317037 and J23100 had cut
 +
 +
 +
-Gel extraction
 +
 +
Gel extracted to DNA refined
 +
 +
 +
Results for concentration
 +
 +
Kenedy 20
 +
 +
Keit 32
 +
 +
Jason 0
 +
 +
Jiro 10
 +
 +
 +
-Digestion
 +
 +
J23100: S and P
 +
 +
P0440: X adn P
 +
 +
 +
-Preculture
 +
 +
Precultured K317037, then incubated at 37 deg C.
 +
 +
 +
Made 50*TAEBuffer

Revision as of 15:38, 5 October 2011

-Visualization-

Ethanol precipitation of J23100,I714051.

Concentration of J23100,I714051 was still low.

@transformation OriTr+GFP and OriT, but failure


-Targeting-

-Plasmid extraction

Used QlaprepSpinMiniprepkit


Results

J23100

Jaiko 123

Jian 82


P0440

Pisuke 158

Pichiku 155

(ng/ul)


-Electrophoresis

Checked for K317037 and J23100


Results

K317037 and J23100 had cut


-Gel extraction

Gel extracted to DNA refined


Results for concentration

Kenedy 20

Keit 32

Jason 0

Jiro 10


-Digestion

J23100: S and P

P0440: X adn P


-Preculture

Precultured K317037, then incubated at 37 deg C.


Made 50*TAEBuffer

Retrieved from "http://2011.igem.org/Team:Tokyo_Metropolitan/Notebook/S02"