-Speeding Up-

Made agar plates

Made each five agar plates contained kanamycin and chloramphenicol.

-Visualization-

Digestion,electrophoresis and gel extraction J23100,I714051.

Confirm plasmid extraction was succeeded

Concentration of DNA solution(J23100,I714051) will measure tomorrow.

@DNA refine(OriTr+GFP,OriT)

electrophoresis and gel extract restriction enzymed sample(8/31).

@ligation

considering a small quantity of refined DNA, change reagent composition.

I. ligate solution

　oriTr:1μl

　oriTr+GFP:12μl

　T4：1μl

　×10 buffer：2μl

II. control solution

　DW:12μl

　oriTr:1μl

　T4：1μl

　×10 buffer：2μl